Introduction of large insert DNA into mammalian cells and embryos

Current Protocols in Human Genetics
R H ReevesB Lamb

Abstract

This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V PachnisF Costantini
Feb 2, 2000·Applied and Environmental Microbiology·L RiemannF Azam

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Citations

Jan 18, 2018·Current Protocols in Molecular Biology·Huntington Potter, Richard Heller
Sep 14, 2011·Current Protocols in Stem Cell Biology·Stuart Avery
Sep 11, 2019·Current Protocols in Molecular Biology·Ameet ShettyFred Winston
Apr 26, 2016·Current Protocols in Molecular Biology·Tineke L Lenstra, Daniel R Larson
Feb 6, 2017·Journal of Dairy Science·Maxwell L Van TassellMichael J Miller

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