PMID: 7568159Oct 10, 1995

Introduction of the transposable element Minos into the germ line of Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
T G LoukerisC Savakis

Abstract

A transposon based on the transposable element Minos from Drosophila hydei was introduced into the genome of Drosophila melanogaster using transformation mediated by the Minos transposase. The transposon carries a wild-type version of the white gene (w) of Drosophila inserted into the second exon of Minos. Transformation was obtained by injecting the transposon into preblastoderm embryos that were expressing transposase either from a Hsp70-Minos fusion inserted into the genome via P-element-mediated transformation or from a coinjected plasmid carrying the Hsp70-Minos fusion. Between 1% and 6% of the fertile injected individuals gave transformed progeny. Four of the insertions were cloned and the DNA sequences flanking the transposon ends were determined. The "empty" sites corresponding to three of the insertions were amplified from the recipient strain by PCR, cloned, and sequenced. In all cases, the transposon has inserted into a TA dinucleotide and has created the characteristic TA target site duplication. In the absence of transposase, the insertions were stable in the soma and the germ line. However, in the presence of the Hsp70-Minos gene the Minos-w transposon excises, resulting in mosaic eyes and germ-line reversion to t...Continue Reading

References

Sep 19, 2009·Genetica·Jeziel D DamascenoLuiz R O Tosi
Apr 14, 2010·Transgenic Research·Holly J FergusonM J Fraser
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Aug 14, 2003·Insect Biochemistry and Molecular Biology·Raymond WilsonD A O'Brochta
Sep 1, 1996·Insect Biochemistry and Molecular Biology·D A O'Brochta, P W Atkinson
Oct 24, 2002·Development, Growth & Differentiation·Hongjie ZhangSumihare Noji
Jan 1, 1997·Annual Review of Biochemistry·N L Craig
Dec 9, 2000·Annual Review of Entomology·P W AtkinsonD A O'Brochta
Dec 8, 2005·Annual Review of Entomology·Peter A Follett, Lisa G Neven
Jan 1, 1997·Annual Review of Genetics·D L HartlE R Lozovskaya
Dec 6, 2007·Genome Biology·Yasunori SasakuraSatoko Awazu
Apr 21, 2010·PloS One·Christopher J Potter, Liqun Luo
Jun 24, 2005·Genetics·Athanasios MetaxakisC Savakis
Sep 15, 2010·Genetics·Thomas W ClineLisa Megna
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anastasios Pavlopoulos, Michalis Averof
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·L ZagoraiouC Savakis
Feb 2, 1999·Insect Molecular Biology·S T ThibaultThomas A Miller
May 16, 2014·Genes·Radoslaw K Ejsmont, Bassem A Hassan
Aug 10, 2007·General and Comparative Endocrinology·Ank ReumerLiliane Schoofs
Oct 25, 2008·Archives of Insect Biochemistry and Physiology·Tamas LukacsovichDaisuke Yamamoto
Jul 16, 2013·Pest Management Science·Thomas A Miller
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Daniel St Johnston
May 30, 2015·Fungal Genetics and Biology : FG & B·Minoas EvangelinosGeorge Diallinas
Apr 12, 2017·Insect Molecular Biology·F ChuMarcé D Lorenzen
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin
Jan 5, 2000·Insect Molecular Biology·S H LeeM G Kidwell
Jan 12, 2007·Molecular Genetics and Genomics : MGG·K UchinoT Tamura
Mar 8, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yasunori Sasakura
Oct 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yasunori SasakuraKazuo Inaba
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joseph M KaminskiMatthew B Ward
Dec 2, 2017·Archives of Insect Biochemistry and Physiology·Fu-Chyun ChuMarcé D Lorenzen

Citations

Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H S SeifertF Heffron
May 1, 1985·Developmental Biology·H Steller, V Pirrotta
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jun 25, 1983·Nucleic Acids Research·B RosenzweigD Hirsh
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G FranzC Savakis

Related Concepts

Fertility
Embryo
Transformation, Genetic
Shuttle Vectors
Transposase
Heat-Shock Proteins 70
Exons
Drosophila
Southern Blot Result
Drosophila hydei

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