Introgression molecular analysis of a leaf rust resistance gene from Coffea liberica into C. arabica L

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
N S PrakashP Lashermes

Abstract

Leaf rust caused by the fungus Hemileia vastatrix is the most devastating disease of arabica coffee ( Coffea arabica). Therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. The purpose of the present work was to gain insight into the mechanism of introgression into C. arabica of a leaf rust resistance gene from C. liberica (i.e. S(H)3 resistance factor) and to identify associated molecular markers. An F(2) progeny (i.e. 101 individuals) derived from a cross between Matari, an arabica accession and liberica-introgressed line S.288, was evaluated for resistance against three different races of H. vastatrix. The progeny segregated for the S(H)3 gene in a 3:1 ratio, as expected for a single dominant gene. Amplified fragment length polymorphism analysis of a population subset using 80 different primer combinations revealed that at least half of the total polymorphism observed in the population is associated with introgression of C. liberica chromosome fragments. Furthermore, 15 primer combinations generating candidate marker bands associated with the S(H)3 resistance gene were used to analyse the whole F(2) population. A total of 34 marker bands originating from ...Continue Reading

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Apr 2, 1999·Molecular & General Genetics : MGG·P LashermesA Charrier
Dec 26, 2001·Molecular Biology and Evolution·Erik RichlyDario Leister
Apr 6, 2002·Science·Stephen A GoffSteven Briggs
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F. AnthonyP. Lashermes
Jan 30, 2007·Journal of the American College of Cardiology·Sripal BangaloreFranz H Messerli

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Citations

Nov 1, 2011·The Journal of Heredity·Marie-Christine CombesPhilippe Lashermes
Sep 26, 2007·Genome Génome / Conseil National De Recherches Canada·Juan Carlos HerreraPhilippe Lashermes
May 18, 2011·BMC Genomics·Alessandra F RibasPhilippe Lashermes
Sep 13, 2012·Biotechnology Research International·M K Mishra, A Slater
Nov 26, 2016·Molecular Plant Pathology·Pedro TalhinhasMaria do Céu Silva

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