Introgression of ivermectin resistance genes into a susceptible Haemonchus contortus strain by multiple backcrossing.

PLoS Pathogens
Elizabeth RedmanJohn S Gilleard

Abstract

Anthelmintic drug resistance in livestock parasites is already widespread and in recent years there has been an increasing level of anthelmintic drug selection pressure applied to parasitic nematode populations in humans leading to concerns regarding the emergence of resistance. However, most parasitic nematodes, particularly those of humans, are difficult experimental subjects making mechanistic studies of drug resistance extremely difficult. The small ruminant parasitic nematode Haemonchus contortus is a more amenable model system to study many aspects of parasite biology and investigate the basic mechanisms and genetics of anthelmintic drug resistance. Here we report the successful introgression of ivermectin resistance genes from two independent ivermectin resistant strains, MHco4(WRS) and MHco10(CAVR), into the susceptible genome reference strain MHco3(ISE) using a backcrossing approach. A panel of microsatellite markers were used to monitor the procedure. We demonstrated that after four rounds of backcrossing, worms that were phenotypically resistant to ivermectin had a similar genetic background to the susceptible reference strain based on the bulk genotyping with 18 microsatellite loci and individual genotyping with a s...Continue Reading

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Citations

Dec 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Andrew C KotzeRoger K Prichard
Apr 30, 2013·Veterinary Parasitology·J L Fitzpatrick
Jun 5, 2015·International Journal for Parasitology. Drugs and Drug Resistance·D J BartleyA A Morrison
Mar 14, 2017·Trends in Parasitology·Roz LaingEileen Devaney
Mar 31, 2018·PLoS Neglected Tropical Diseases·Mostafa ZamanianErik C Andersen
Dec 7, 2017·Frontiers in Cellular and Infection Microbiology·Victoria GillanEileen Devaney
Aug 16, 2019·Parasites & Vectors·Simone Cristina Méo NiciuraAlessandro Francisco Talamini do Amarante
Sep 9, 2017·Frontiers in Microbiology·Chunqun WangMin Hu
Oct 25, 2020·Trends in Parasitology·Nichola Eliza Davies Calvani, Jan Šlapeta
Dec 12, 2020·International Journal for Parasitology. Drugs and Drug Resistance·Andrew C KotzeRoger K Prichard

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis
PCA
RNAseq

Software Mentioned

GenAlEx
BootStreat
Arlequin
Genemapper

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