Introgression of leginsulin, a cysteine-rich protein, and high-protein trait from an Asian soybean plant introduction genotype into a North American experimental soybean line

Journal of Agricultural and Food Chemistry
Hari B KrishnanRandall L Nelson

Abstract

Soybean is an important protein source for both humans and animals. However, soybean proteins are relatively poor in the sulfur-containing amino acids, cysteine and methionine. Improving the content of endogenous proteins rich in sulfur-containing amino acids could enhance the nutritive value of soybean meal. Leginsulin, a cysteine-rich peptide, predominantly accumulates in Asian soybean accessions but not in most North American cultivars. By screening diverse soybean accessions from the USDA Soybean Germplasm Collection, we were able to identify one plant introduction, PI 427138, as a high-protein line with relatively high amounts of both elemental sulfur and leginsulin. We introgressed these desirable traits from PI 427138 into an experimental line with the aim of improving the overall protein content and quality of seed proteins. Biochemical characterization of inbred progenies from the cross of LD00-3309 with PI 427138 grown at six locations revealed stable ingression of high protein, high elemental sulfur, and high leginsulin accumulation. Comparison of soybean seed proteins resolved by high-resolution 2-D gel electrophoresis in combination with Delta2D image analysis software revealed preferential accumulation of a few gl...Continue Reading

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Feb 25, 2011·Journal of Agricultural and Food Chemistry·Hari B Krishnan, Randall L Nelson
Mar 29, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mehrzad EskandariIstvan Rajcan
Jan 3, 2014·BMC Genomics·Eun-Young HwangPerry B Cregan

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Citations

Dec 11, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jeffrey D BoehmZenglu Li
Aug 13, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gunvant PatilHenry T Nguyen
Apr 2, 2021·Journal of Proteomics·Hisashi Hirano

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