Introgression of UfCyt c6 , a thylakoid lumen protein from a green seaweed Ulva fasciata Delile enhanced photosynthesis and growth in tobacco

Molecular Biology Reports
Sweta K YadavBhavanath Jha

Abstract

Cytochromes are important components of photosynthetic electron transport chain. Here we report on genetic transformation of Cytochrome c6 (UfCyt c6) gene from Ulva fasciata Delile in tobacco for enhanced photosynthesis and growth. UfCyt c6 cDNA had an open reading frame of 330 bp encoding a polypeptide of 109 amino acids with a predicted molecular mass of 11.65 kDa and an isoelectric point of 5.21. UfCyt c6 gene along with a tobacco petE transit peptide sequence under control of CaMV35S promoter was transformed in tobacco through Agrobacterium mediated genetic transformation. Transgenic tobacco grew normal and exhibited enhanced growth as compared to wild type (WT) and vector control (VC) tobacco. Transgenic tobacco had higher contents of photosynthetic pigments and better ratios of photosynthetic pigments. The tobacco expressing UfCyt c6 gene exhibited higher photosynthetic rate and improved water use efficiency. Further activity of the water-splitting complex, photosystem II quantum yield, photochemical quenching, electron transfer rate, and photosynthetic yield were found comparatively higher in transgenic tobacco as compared to WT and VC tobacco. Alternatively basal quantum yield of non-photochemical processes in PSII and ...Continue Reading

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Citations

Feb 18, 2019·Journal of Experimental Botany·Andrew J SimkinChristine A Raines
Dec 6, 2019·The Plant Journal : for Cell and Molecular Biology·Andrew J SimkinTracy Lawson
Jul 2, 2020·Plants·Lisa RotaspertiPaolo Pesaresi
Aug 13, 2020·Nature Plants·Patricia E López-CalcagnoChristine A Raines
Dec 29, 2020·Plant Communications·Willian Batista-SilvaWagner L Araújo
Jun 15, 2021·Frontiers in Microbiology·Sanjana KaulManoj K Dhar
Jul 9, 2021·Photosynthesis Research·Sajad HussainWeiguo Liu
Aug 8, 2021·International Journal of Molecular Sciences·Fahimeh ShahinniaMohammad-Reza Hajirezaei
Oct 9, 2021·Biochemical Society Transactions·Concepción IñiguezJeroni Galmés

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Datasets Mentioned

BETA
KT184330

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

TMpred
ClustalW
ScanProsite
NetPhos
Motif
MultiLoc2
MEGA
ProtParam
BLAST
PSIPRED

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