Intron loss from the NADH dehydrogenase subunit 4 gene of lettuce mitochondrial DNA: evidence for homologous recombination of a cDNA intermediate

Molecular & General Genetics : MGG
K T GeissC A Makaroff

Abstract

The mitochondrial gene coding for subunit 4 of the NADH dehydrogenase complex I (nad4) has been isolated and characterized from lettuce, Lactuca sativa. Analysis of nad4 genes in a number of plants by Southern hybridization had previously suggested that the intron content varied between species. Characterization of the lettuce gene confirms this observation. Lettuce nad4 contains two exons and one group IIA intron, whereas previously sequenced nad4 genes from turnip and wheat contain three group IIA introns. Northern analysis identified a transcript of 1600 nucleotides, which represents the mature nad4 mRNA and a primary transcript of 3200 nucleotides. Sequence analysis of lettuce and turnip nad4 cDNAs was used to confirm the intron/exon border sequences and to examine RNA editing patterns. Editing is observed at the 5' and 3' ends of the lettuce transcript, but is absent from sequences that correspond to exons two, three and the 5' end of exon four in turnip and wheat. In contrast, turnip transcripts are highly edited in this region, suggesting that homologous recombination of an edited and spliced cDNA intermediate was involved in the loss of introns two and three from an ancestral lettuce nad4 gene.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1992·Trends in Genetics : TIG·B WissingerW Schuster
Mar 1, 1991·Current Genetics·K D Pruitt, M R Hanson
Nov 1, 1991·Plant Molecular Biology·G C Ward, C S Levings
Jul 11, 1991·Nucleic Acids Research·R T D'AquilaJ C Kaplan
Apr 1, 1990·Trends in Genetics : TIG·J D Palmer
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J A WahleithnerD R Wolstenholme
May 1, 1989·Molecular & General Genetics : MGG·E Levra-JuilletG Faye
Jan 1, 1989·Annual Review of Cell Biology·M W Gray
Mar 1, 1985·Cell·J D BoekeG R Fink
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R Kolodner, K K Tewari
Jan 1, 1993·Annual Review of Biochemistry·A M Lambowitz, M Belfort
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M W Gray, P S Covello
Jan 1, 1989·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M ShirzadeganJ D Palmer

❮ Previous
Next ❯

Citations

Oct 1, 1996·Plant Molecular Biology·R M MaierJ M Grienenberger
Apr 10, 2012·Journal of Molecular Evolution·Argelia CuencaAnne Hoppe Jahren
Oct 10, 2006·Genetica·Frédérique Maczkowiak, Jean-Luc Da Lage
Jun 3, 1996·Biochimica Et Biophysica Acta·G J Arts, R Benne
Jul 17, 1996·Biochimica Et Biophysica Acta·G L Sper-WhitisJ C Vaughn
Oct 27, 1995·Gene·J FitzgibbonD M Hunt
May 30, 2001·Trends in Genetics : TIG·L Bonen, J Vogel
Jun 6, 2008·Molecular Biology and Evolution·M Virginia Sanchez-PuertaJeffrey D Palmer
Jan 10, 2012·Genome Génome / Conseil National De Recherches Canada·Hiroyo KagamiTomohiko Kubo
Oct 1, 1996·Journal of Molecular Evolution·J L Da LageM L Cariou
May 26, 2017·Molecular Biology and Evolution·Zhiqiang WuHan Chuan Ong
May 16, 2018·Plant Molecular Biology·Alejandro A EderaM Virginia Sanchez-Puerta
May 17, 2000·American Journal of Botany·F T BakkerM Gibby
Jul 21, 2016·Genome Biology and Evolution·Argelia CuencaGitte Petersen
Jun 21, 2019·International Journal of Molecular Sciences·Kamil MyszczyńskiJakub Sawicki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.