Intronic hammerhead ribozymes are ultraconserved in the human genome.

EMBO Reports
Marcos de la Peña, Inmaculada García-Robles

Abstract

Small ribozymes have been regarded as living fossils of a prebiotic RNA world that would have remained in the genomes of modern organisms. In this study, we report the ultraconserved occurrence of hammerhead ribozymes in Amniota genomes (reptiles, birds and mammals, including humans), similar to those described previously in amphibians and platyhelminth parasites. The ribozymes mapped to intronic regions of different genes, such as the tumour suppressor RECK in birds and mammals, a mammalian tumour antigen and the dystrobrevin beta in lizards and birds. In vitro characterization confirmed a high self-cleavage activity, whereas analysis of RECK-expressed sequence tags revealed fusion events between the in vivo self-cleaved intron and U5 or U6 small nuclear RNA fragments. Together, these results suggest a conserved role for these ribozymes in messenger RNA biogenesis.

References

Feb 13, 1987·Cell·L M Epstein, J G Gall
May 1, 1994·Japanese Journal of Cancer Research : Gann·T Kuroki
Feb 12, 1998·The Journal of Biological Chemistry·M F PetersS C Froehner
Jun 25, 1998·Molecular and Cellular Biology·G FerbeyreR Cedergren
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·C TakahashiM Noda
Jul 19, 2000·Genome Research·G FerbeyreR Cedergren
Jul 26, 2003·Nature Structural Biology·Anastasia KhvorovaSumedha D Jayasena
Mar 5, 2004·The British Journal of Dermatology·T B HartmannS Eichmüller
Jun 7, 2005·The Plant Cell·Rita PrzybilskiChristian Hammann
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·R Mark GradyJoshua R Sanes
Mar 18, 2006·Molecular Cell·Michael J DyeNick J Proudfoot
Sep 23, 2006·Science·Kourosh Salehi-AshtianiJack W Szostak
Mar 28, 1986·Science·G A ProdyG Bruening
Dec 8, 2007·Advances in Parasitology·David A Mayer, Bernard Fried
Jul 11, 2008·Nature·Monika MartickWilliam G Scott
Jul 17, 2009·Nature·Matthew BerrimanNajib M El-Sayed
Dec 8, 2009·Science·Chiu-Ho T WebbAndrej Lupták
Aug 14, 2010·RNA·Marcos de la Peña, Inmaculada García-Robles

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Citations

Nov 11, 2011·Journal of the American Chemical Society·Erich G Chapman, Victoria J DeRose
Jan 25, 2011·The Journal of Biological Chemistry·Randi M JimenezAndrej Lupták
Oct 26, 2011·Biochemistry. Biokhimii︠a︡·C P Godfried Sie, M Kuchka
Mar 29, 2012·RNA·Christian HammannMarcos de la Peña
Aug 14, 2010·RNA·Marcos de la Peña, Inmaculada García-Robles
Nov 18, 2010·RNA·Carsten SeehaferChristian Hammann
May 17, 2011·PLoS Computational Biology·Jonathan PerreaultRonald R Breaker
Feb 9, 2012·International Journal of Molecular Sciences·Daniel Frías-Lasserre
Apr 30, 2014·Nature Reviews. Genetics·Kevin V Morris, John S Mattick
Mar 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Miriam SkilandatRoland K O Sigel
Jul 21, 2014·Nature Chemical Biology·Yijin LiuDavid M J Lilley
Aug 20, 2011·Wiley Interdisciplinary Reviews. RNA·Katarzyna Matylla-KulinskaRenée Schroeder
Jul 8, 2015·Chembiochem : a European Journal of Chemical Biology·Shu-ichi NakanoNaoki Sugimoto
Aug 20, 2014·Molecular Biology and Evolution·Amelia Cervera, Marcos De la Peña
Jan 15, 2014·Chemical Reviews·Sethuramasundaram PitchiayaNils G Walter
Jan 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcos de la PeñaAmelia Cervera
Oct 21, 2015·Trends in Biochemical Sciences·Randi M JimenezAndrej Lupták
Apr 28, 2017·RNA Biology·Marcos de la Peña, Amelia Cervera
Jan 10, 2018·Frontiers in Microbiology·Daniel Frías-Lasserre, Cristian A Villagra
Mar 22, 2020·Nucleic Acids Research·Amelia Cervera, Marcos de la Peña
Sep 11, 2019·Nucleic Acids Research·Christina E WeinbergChristian Hammann
Mar 25, 2019·Viruses·Marie-Christine MaurelGiuseppe Zaccai
Mar 24, 2021·Nature Chemical Biology·Yue ChenPhilipp Kapranov
Mar 24, 2021·Nature Chemical Biology·Claire C Chen, Andrej Lupták
Mar 18, 2021·BMC Molecular and Cell Biology·Aiyada AroonsriPhilip James Shaw
May 6, 2021·Wiley Interdisciplinary Reviews. RNA·Timothy J Wilson, David M J Lilley
Jul 10, 2021·Chembiochem : a European Journal of Chemical Biology·Shu-Ichi NakanoNaoki Sugimoto

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