Introns, alternative splicing, spliced leader trans-splicing and differential expression of pcna and cyclin in Perkinsus marinus

Protist
Huan ZhangSenjie Lin

Abstract

To gain understanding on the structure and regulation of growth-related genes of the parasitic alveolatePerkinsus marinus, we analyzed genes encoding proliferating cell nuclear antigen (pcna) and cyclins (cyclin). Comparison of the full-length cDNAs with the corresponding genomic sequences revealedtrans-splicing of the mRNAs of these genes with a conserved 21-22 nt spliced leader. Over 10 copies ofpcnawere detected, with identical gene structures and similar nucleotide (nt) sequences (88-99%), encoding largely identical amino acid sequences (aa). Two distinct types ofcyclin(Pmacyclin1 andPmacyclin2) were identified, with 66-69% nt and 81-85% aa similarities.Pmacyclin2 was organized in tandem repeats, and was alternatively spliced, giving rise to five subtypes of transcripts. For bothpcnaandcyclingenes, 6-10 introns were found. Quantitative RT-PCR assays showed thatpcnaandPmacyclin2 expression levels were low with small variations during a 28-h time course, whereasPmacyclin1 transcript abundance was 10-100 times higher, and increased markedly during active cell division, suggesting that it is a mitoticcyclinand can be a useful growth marker for this species. The gene structure and expression features along with phylogenetic resu...Continue Reading

Citations

Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mathieu BeaucheminDavid Morse
Feb 26, 2013·PloS One·Christopher J Jackson, Ross F Waller
Sep 6, 2011·International Journal for Parasitology·José A Fernández RobledoGerardo R Vasta
Mar 26, 2011·The Journal of Eukaryotic Microbiology·Jennifer L Hearne, Joseph S Pitula
May 30, 2015·Journal of Invertebrate Pathology·Marta Gómez-ChiarriDina Proestou
Apr 26, 2011·Research in Microbiology·Senjie Lin
Feb 22, 2012·European Journal of Protistology·Américo MarquesM Leonor Teles-Grilo
Sep 25, 2012·European Journal of Protistology·Sandra M Casas, Jerome F La Peyre
Nov 1, 2013·Microorganisms·Sougata Roy, David Morse
May 7, 2016·PloS One·Emma R ColdJosé A Fernández Robledo

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