Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles

Plants
Oleg GorshkovT A Gorshkova

Abstract

Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from 23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, and miR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phlo...Continue Reading

References

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A ImamuraT Mizuno
Feb 28, 2002·Phytochemistry·Michael ReicheltJonathan Gershenzon
Oct 8, 2004·Molecular Cell·Franck VazquezPatrice Crété
Apr 26, 2005·The Plant Cell·Michael J Axtell, David P Bartel
Jun 18, 2005·The Plant Journal : for Cell and Molecular Biology·Gorou HoriguchiHirokazu Tsukaya
Oct 11, 2005·Planta·M V AgeevaA A M van Lammeren
Jun 29, 2006·Journal of Experimental Botany·Xiaoning CaiYajun Wu
Feb 26, 2009·Cell·Olivier Voinnet
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Dec 19, 2009·Plant Physiology·Réjane PratelliGuillaume Pilot
Dec 22, 2009·Development·Ramiro E RodriguezJavier F Palatnik
Nov 11, 2011·Wiley Interdisciplinary Reviews. RNA·Shobha Vasudevan
Nov 24, 2011·Nucleic Acids Research·David M GoodsteinDaniel S Rokhsar
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Mar 24, 2012·PloS One·Joshua R PuzeyElena M Kramer
Jul 5, 2012·The Plant Journal : for Cell and Molecular Biology·Zhiwen WangMichael K Deyholos
Jul 31, 2012·Journal of Plant Physiology·Godfrey NeutelingsSimon Hawkins
Aug 15, 2012·Mechanisms of Development·Martin A MecchiaJavier F Palatnik
Nov 20, 2012·Journal of Experimental Botany·Mathias SchuetzBrian Ellis
Apr 22, 2014·Biochemical and Biophysical Research Communications·Wangxiong HuJian-Hong Xu
May 8, 2014·Journal of Experimental Botany·Gang LiangDiqiu Yu
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Nov 16, 2014·Nucleic Acids Research·Xin YiZhen Su
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Dec 18, 2015·F1000Research·Michael I LoveWolfgang Huber
Jan 7, 2016·Frontiers in Plant Science·Gea GuerrieroInés Ezcurra
Feb 13, 2016·The Plant Cell·Xiaofeng Fang, Yijun Qi

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Citations

Dec 8, 2020·Frontiers in Genetics·Dmitry GalinouskyTatyana Gorshkova

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Datasets Mentioned

BETA
PRJNA475325

Methods Mentioned

BETA
transgenic
RNA-Seq
PCR
PCA

Software Mentioned

gplots R package
bowtie2
HISAT2
samtools
R
DESeq2
count
R package
DESeq
HTSeq

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