Inula Viscosa Extracts Induces Telomere Shortening and Apoptosis in Cancer Cells and Overcome Drug Resistance

Nutrition and Cancer
Nawal MerghoubHamid Morjani

Abstract

Telomerase is activated in human papillomavirus (HPV) positive cervical cancer and targeting telomeres offers a novel anticancer therapeutic strategy. In this study, the telomere targeting properties, the cytotoxic as well as the pro-apoptotic effects of hexane (IV-HE) and dichloromethane (IV-DF) fractions from Inula viscosa L. extracts were investigated on human cervical HeLa and SiHa cancer cells. Our data demonstrate that IV-HE and IV-DF extracts were able to inhibit cell growth in HeLa and SiHa cells in a dose-dependent manner and studied resistant cell lines exhibited a resistance factor less than 2 when treated with the extracts. IV-HE and IV-DF extracts were able to inhibit telomerase activity and to induce telomere shortening as shown by telomeric repeat amplification protocol and TTAGGG telomere length assay, respectively. The sensitivity of fibroblasts to the extracts was increased when telomerase was expressed. Finally, IV-HE and IV-DF were able to induce apoptosis as evidenced by an increase in annexin-V labeling and caspase-3 activity. This study provides the first evidence that the IV-HE and IV-DF extracts from Inula viscosa L. target telomeres induce apoptosis and overcome drug resistance in tumor cells. Future s...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Apr 22, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D K ZhangS W Tsao
Sep 14, 2001·Human Molecular Genetics·M A CeroneS Bacchetti
Oct 24, 2002·Biological & Pharmaceutical Bulletin·Tenji KonishiTakao Konoshima
Apr 26, 2003·Natural Product Research·Musa H Abu Zarga Atta-Ur-Rahman
Aug 18, 2005·Phytotherapy Research : PTR·Nikolay A SpiridonovVladimir V Arkhipov
Aug 23, 2005·Cancer Letters·Pei-Rong HuangTzu-Chien V Wang
Aug 17, 2006·Phytotherapy Research : PTR·David C DornAugust Dorn
May 15, 2007·The New England Journal of Medicine·Jan M Agosti, Sue J Goldie
Jun 26, 2007·Biochemical and Biophysical Research Communications·David SadavaSusan E Kane
Jul 27, 2007·Natural Product Research·Gianfranco FontanaMaria Pia Paternostro
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lloyd Kelland
Sep 8, 2007·Biochimie·Anne De CianJean-Louis Mergny
Oct 9, 2007·Biochemical Pharmacology·Sharon RozenblatSara Dovrat
Sep 30, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Cheol ParkYung Hyun Choi
Dec 24, 2010·Scientia pharmaceutica·Wamidh H Talib, Adel M Mahasneh
Nov 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zdenka KucekovaPetr Saha
Mar 16, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wamidh H TalibAdel M Mahasneh
Aug 11, 2012·Mini Reviews in Medicinal Chemistry·Yoshiyuki MizushinaHiromi Yoshida
Feb 5, 2013·Genes & Nutrition·Agnes A Michalczyk, M Leigh Ackland
May 29, 2013·Virology·Scott B Vande Pol, Aloysius J Klingelhutz

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Citations

Dec 7, 2016·Journal of Cellular Biochemistry·Nawel MerghoubMohamed El Mzibri
Feb 6, 2020·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Patrizia VirdisLuigi Podda
Jul 12, 2017·Frontiers in Pharmacology·Jorge M Alves-SilvaLígia Salgueiro
Dec 27, 2018·ACS Omega·Navakoon KaewtunjaiWirote Tuntiwechapikul

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