Invading the yeast nucleus: a nuclear localization signal at the C terminus of Ty1 integrase is required for transposition in vivo.

Molecular and Cellular Biology
M A KennaJ D Boeke

Abstract

Retrotransposon Ty1 faces a formidable cell barrier during transposition--the yeast nuclear membrane which remains intact throughout the cell cycle. We investigated the mechanism by which transposition intermediates are transported from the cytoplasm (the presumed site of Ty1 DNA synthesis) to the nucleus, where they are integrated into the genome. Ty1 integrase has a nuclear localization signal (NLS) at its C terminus. Both full-length integrase and a C-terminal fragment localize to the nucleus. C-terminal deletion mutants in Ty1 integrase were used to map the putative NLS to the last 74 amino acid residues of integrase. Mutations in basic segments within this region decreased retrotransposition at least 50-fold in vivo. Furthermore, these mutant integrase proteins failed to localize to the nucleus. Production of virus-like particles, reverse transcriptase activity, and complete in vitro Ty1 integration resembled wild-type levels, consistent with failure of the mutant integrases to enter the nucleus.

References

Jan 1, 1975·International Review of Cytology·D F Kubai
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M I BukrinskyM Stevenson
Sep 1, 1991·Journal of Virology·D J GarfinkelT D Copeland
Jan 1, 1991·Methods in Enzymology·A E Adams, J R Pringle
Mar 1, 1988·The Journal of Cell Biology·S I Dworetzky, C M Feldherr
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H Xu, J D Boeke
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J J ClareP J Farabaugh
Jan 25, 1989·Nucleic Acids Research·F ValletteM Adesnik
Mar 1, 1985·Cell·J D BoekeG R Fink
Oct 1, 1988·Molecular and Cellular Biology·C V Dang, W M Lee
Dec 1, 1995·Journal of Virology·V LauermannJ D Boeke
Sep 1, 1994·Molecular and Cellular Biology·L T Braiterman, J D Boeke
Feb 20, 1995·Virology·J L BrookmanK Gull
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M I BukrinskyM Stevenson
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N K HeinzingerM Emerman
Sep 1, 1994·Molecular and Cellular Biology·L T Braiterman, J D Boeke
Mar 15, 1996·Science·D Görlich, I W Mattaj

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Citations

Sep 25, 2012·Traffic·Mary Ann CheckleyDavid J Garfinkel
Dec 10, 2015·Current Genetics·David J GarfinkelKatarzyna J Purzycka
Jun 11, 2009·IUBMB Life·Laura M McLane, Anita H Corbett
Sep 17, 2013·Current Opinion in Virology·Alan H Schulman
Jul 10, 1998·Virology·G R Whittaker, A Helenius
Oct 10, 2015·PLoS Genetics·Jessica M TuckerDavid J Garfinkel
May 22, 2007·Ageing Research Reviews·Matt Kaeberlein, R Wilson Powers
Nov 2, 2002·Molecular Biology and Evolution·Brooke D Peterson-Burch, Daniel F Voytas
Mar 31, 2015·PLoS Genetics·Krystina L HoVivien Measday
Aug 12, 2005·Cytogenetic and Genome Research·F-X WilhelmA Gabriel
Jan 29, 2000·Molecular Microbiology·D Conte, M J Curcio
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·S RashkovaM-L Pardue
Aug 3, 2010·Molecular Genetics and Genomics : MGG·Anna A FriedlFriederike Eckardt-Schupp
Jun 12, 2019·Cells·Tanner M TessierJoe S Mymryk
Feb 3, 1998·Molecular and Cellular Biology·S P MooreD J Garfinkel
Aug 6, 2008·Molecular and Cellular Biology·Francisco Malagon, Torben Heick Jensen
Oct 18, 2001·Molecular and Cellular Biology·S S LinS B Sandmeyer
Apr 26, 2018·PLoS Genetics·Paul A RowleySara L Sawyer
Sep 29, 2006·Journal of Virology·David J GarfinkelStephen H Hughes
Dec 16, 1998·Journal of Virology·M H Nymark-McMahon, S B Sandmeyer
Jun 22, 2000·Yeast·J F Roth
Jul 3, 2009·Journal of Virology·Sharon P Moore, David J Garfinkel

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