Invasion of differentiated intestinal Caco-2 cells is a sporadic property among atypical enteropathogenic Escherichia coli strains carrying common intimin subtypes

Pathogens and Disease
Veronica C R PachecoT A Gomes

Abstract

Atypical enteropathogenic Escherichia coli (aEPEC) strains produce attaching-effacing (AE) lesions on enterocytes due to the interaction of the adhesin intimin with its translocated receptor. aEPEC strain 1551-2 was previously shown to invade HeLa and T84 cells by means of the uncommon intimin subtype omicron. Other aEPEC strains carrying uncommon intimin subtypes have also been shown to invade differentiated T84 intestinal cells. In this study, seven aEPEC strains carrying the most common EPEC intimin subtypes (alpha, beta, and gamma) were evaluated regarding the ability to invade differentiated intestinal Caco-2 cells. Although all strains adhered to and promoted AE lesions, the numbers of cell-associated bacteria varied significantly between the different strains regardless of the intimin subtype (P < 0.05). Gentamicin protection assay and transmission electron microscopy analyses showed that in comparison with the invasive strain 1551-2, only one strain (aEPEC EC423/03, intimin beta) was invasive (P = 0.05). Although both strains persisted intracellularly until 48 h, the number of viable bacteria of EC423/03 decreased, whereas that of 1551-2 increased significantly up to 24 h and then decreased. In conclusion, invasiveness ...Continue Reading

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Citations

Sep 27, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·André Pitondo-SilvaRodrigo Tavanelli Hernandes
Mar 3, 2018·Genome Announcements·Fabiano Teodoro RomãoTânia Aparecida Tardelli Gomes
Nov 22, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Tânia A T GomesMarina B Martinez
Dec 6, 2018·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Vivian PeiranoGustavo Varela
Mar 1, 2020·Communications Biology·Tetsuya IkedaTetsuya Hayashi
Jan 5, 2021·Frontiers in Cellular and Infection Microbiology·Fabiano T RomãoTânia A T Gomes
Jun 27, 2020·EcoSal Plus·Tânia A T GomesTetsuya Hayashi

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