Invasion of shoot apical meristems by Chrysanthemum stunt viroid differs among Argyranthemum cultivars

Frontiers in Plant Science
Zhibo ZhangDag-Ragnar Blystad

Abstract

Chrysanthemum stunt viroid (CSVd) is a damaging pathogen attacking Argyranthemum plants. Our study attempted to reveal distribution patterns of CSVd in shoot apical meristems (SAM) and to explore reasons for differential ability of CSVd to invade SAM of selected Argyranthemum cultivars. Symptom development was also observed on greenhouse-grown Argyranthemum plants. Viroid localization using in situ hybridization revealed that the ability of CSVd to invade SAM differed among cultivars. In diseased 'Yellow Empire' and 'Butterfly', CSVd was found in all tissues including the uppermost cell layers in the apical dome (AD) and the youngest leaf primordia 1 and 2. In diseased 'Border Dark Red' and 'Border Pink', CSVd was detected in the lower part of the AD and elder leaf primordia, leaving the upper part of the AD, and leaf primordia 1 and 2 free of viroid. Histological observations and transmission electron microscopy showed similar developmental patterns of vascular tissues and plasmodesmata (PD) in the SAM of 'Yellow Empire' and 'Border Dark Red', while immunolocalization studies revealed a major difference in the number of callose (β-1, 3-glucan) particles deposited at PD in SAM. A lower number of callose particles were found dep...Continue Reading

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Citations

Oct 17, 2018·Plant Methods·Min-Rui WangQiao-Chun Wang
Oct 28, 2017·Frontiers in Plant Science·Mazen Alazem, Na-Sheng Lin

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Methods Mentioned

BETA
PCR
in vitro transcription
dot blot

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