Invasion of Wolbachia into Anopheles and other insect germlines in an ex vivo organ culture system.

PloS One
Grant L HughesJason L Rasgon

Abstract

The common bacterial endosymbiont Wolbachia manipulates its host's reproduction to promote its own maternal transmission, and can interfere with pathogen development in many insects making it an attractive agent for the control of arthropod-borne disease. However, many important species, including Anopheles mosquitoes, are uninfected. Wolbachia can be artificially transferred between insects in the laboratory but this can be a laborious and sometimes fruitless process. We used a simple ex vivo culturing technique to assess the suitability of Wolbachia-host germline associations. Wolbachia infects the dissected germline tissue of multiple insect species when the host tissue and bacteria are cultured together. Ovary and testis infection occurs in a density-dependent manner. Wolbachia strains are more capable of invading the germline of their native or closely related rather than divergent hosts. The ability of Wolbachia to associate with the germline of novel hosts is crucial for the development of stably-transinfected insect lines. Rapid assessment of the suitability of a strain-host combination prior to transinfection may dictate use of a particular Wolbachia strain. Furthermore, the cultured germline tissues of two major Anoph...Continue Reading

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Citations

Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Grant L HughesJason L Rasgon
Sep 14, 2015·Current Opinion in Virology·Shivanand HegdeGrant L Hughes
Jan 15, 2013·Archives of Insect Biochemistry and Physiology·Xiaoqian ZhangDavid Stanley
Jul 11, 2014·PLoS Neglected Tropical Diseases·Brittany L DodsonJason L Rasgon
Feb 4, 2014·Scientific Reports·Courtney C MurdockMatthew B Thomas
Jan 15, 2017·Applied and Environmental Microbiology·Pamela M WhiteWilliam Sullivan
Jul 28, 2016·MicrobiologyOpen·Jose E PietriWilliam Sullivan
Jun 26, 2020·Frontiers in Microbiology·Mariana MateosGeorge Tsiamis
Jul 30, 2021·Frontiers in Microbiology·Blaire StevenDoug E Brackney

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Methods Mentioned

BETA
transinfection
confocal microscopy
dissection
transinfections
PCR
Fluorescence

Software Mentioned

Photoshop
LSM
Qgene
Zeiss

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