Invasion profiles of Brazilian field isolates of Plasmodium falciparum: phenotypic and genotypic analyses

Infection and Immunity
Cheryl-Ann LoboSara Lustigman

Abstract

The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity reflects the parasite invasion arsenal in the field. We have studied the invasion phenotypes of 14 distinct field isolates from the Legal Amazon areas of Brazil by using erythrocyte invasion assays to investigate invasion into normal, enzyme-treated, and clinical-mutant RBCs. Analysis of these isolates revealed four distinct invasion profiles. Using En(a-) cells to get an unequivocal estimate of the use of glycophorin A (GPA) as a receptor, we found that the 175-kDa erythrocyte-binding antigen (EBA-175)/GPA pathway was used by a minority of the parasite isolates studied. Although polymorphism of region II domains at specific amino acid positions in both EBA-140 and EBA-181 was found in these field isolates, this did not correlate with invasion profiles and thus receptor selectivity. These studies have further confirmed the existence of a significant diversity of invasion pathways in nature and suggest that additional pa...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J H AdamsL H Miller
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A SmytheR F Anders
Aug 1, 1990·The Journal of Clinical Investigation·S A DolanT E Wellems
Jan 1, 1988·Molecular and Biochemical Parasitology·M E Perkins, E H Holt
Mar 1, 1994·Molecular and Biochemical Parasitology·S A DolanL H Miller
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C MayerL H Miller
May 30, 2001·Trends in Parasitology·J H AdamsD S Peterson
Jan 30, 2002·Molecular and Biochemical Parasitology·David L NarumB Kim Lee Sim
Dec 4, 2002·The Journal of Experimental Medicine·D C Ghislaine MayerLouis H Miller
Mar 26, 2003·Infection and Immunity·Jake BaumDavid J Conway
Apr 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manoj T DuraisinghAlan F Cowman
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·D C Ghislaine MayerLouis H Miller

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Citations

Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Wai-Hong ThamAlan F Cowman
Jan 16, 2007·Clinical Microbiology Reviews·David J Conway
May 2, 2007·Infection and Immunity·Cameron V JenningsManoj T Duraisingh
Apr 18, 2007·Infection and Immunity·Anne-Marie DeansJ Alexandra Rowe
Nov 14, 2013·Cellular and Molecular Life Sciences : CMLS·Dawn M WalkerAbhay R Satoskar
Dec 20, 2011·Trends in Parasitology·Wai-Hong ThamAlan F Cowman
Jan 5, 2016·Trends in Parasitology·UNKNOWN WAMIN consortium authors include (in alphabetical order):Zenon A Zenonos
Jul 17, 2007·Molecular Microbiology·Jayasree IyerPeter R Preiser
Sep 28, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michel TheronJulian C Rayner
Jul 14, 2006·Molecular and Biochemical Parasitology·Federica VerraDavid J Conway
Apr 4, 2015·The Journal of Infectious Diseases·Henrietta E Mensah-BrownGordon A Awandare
Jun 26, 2012·International Journal for Parasitology·Amy K Bei, Manoj T Duraisingh
Sep 23, 2008·Trends in Parasitology·Alfred Cortés
Jul 31, 2012·Asian Pacific Journal of Tropical Medicine·Kuppusamy Chenniappan, Sarah H Johns
May 23, 2019·Briefings in Functional Genomics·Oriol Llorà-BatlleAlfred Cortés
Jun 19, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristina E M PerssonJames G Beeson
Apr 11, 2018·Scientific Reports·Gordon A AwandareJosé A Stoute
Mar 31, 2021·Scientific Reports·Laty G ThiamGordon A Awandare

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