Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Journal of Visualized Experiments : JoVE
Jiang RenPeter Ten Dijke

Abstract

In many cases, cancer patients do not die of a primary tumor, but rather because of metastasis. Although numerous rodent models are available for studying cancer metastasis in vivo, other efficient, reliable, low-cost models are needed to quickly access the potential effects of (epi)genetic changes or pharmacological compounds. As such, we illustrate and explain the feasibility of xenograft models using human breast cancer cells injected into zebrafish embryos to support this goal. Under the microscope, fluorescent proteins or chemically labeled human breast cancer cells are transplanted into transgenic zebrafish embryos, Tg (fli:EGFP), at the perivitelline space or duct of Cuvier (Doc) 48 h after fertilization. Shortly afterwards, the temporal-spatial process of cancer cell invasion, dissemination, and metastasis in the living fish body is visualized under a fluorescent microscope. The models using different injection sites, i.e., perivitelline space or Doc are complementary to one another, reflecting the early stage (intravasation step) and late stage (extravasation step) of the multistep metastatic cascade of events. Moreover, peritumoral and intratumoral angiogenesis can be observed with the injection into the perivitelline...Continue Reading

Citations

Nov 7, 2019·Cell Communication and Signaling : CCS·Ulrike NeckmannToril Holien
Oct 17, 2019·Cold Spring Harbor Perspectives in Medicine·Katy R Astell, Dirk Sieger
Nov 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Matthias BaroneRonald Kühne
Jun 3, 2021·International Journal of Molecular Sciences·Ram Manohar BasnetMaurizio Memo
Oct 3, 2018·Molecular Pharmaceutics·Fabiola PortaHenriette E Meyer Zu Schwabedissen
Oct 14, 2021·Cell Death & Disease·Mahsa Shahidi DadrasAristidis Moustakas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Related Papers

Nature Protocols
Stefania Nicoli, Marco Presta
Proceedings of the National Academy of Sciences of the United States of America
Samantha Lin Chiou LeeYihai Cao
Expert Opinion on Drug Discovery
Hannah K BrownTimothy Ja Chico
© 2022 Meta ULC. All rights reserved