Invasive growth of Aspergillus oryzae in rice koji and increase of nuclear number

Fungal biology and biotechnology
Mizuki YasuiNorio Takeshita

Abstract

'Rice koji' is a solid culture of Aspergillus oryzae on steamed rice grains. Multiple parallel fermentation, wherein saccharification of rice by A. oryzae and alcohol fermentation by the budding yeast occur simultaneously, leads to the formation of a variety of ingredients of Japanese sake. In sake brewing, the degree of mycelial invasive growth into the steamed rice, called 'haze-komi', highly correlates with the digestibility and quality of rice koji, since the hyphae growing into the rice secrete amylases and digest starch. In this study, we investigated mycelial distribution of GFP-tagged A. oryzae in rice koji made with different types of rice, such as sake rice and eating rice, with 50 or 90% polishing rate to remove abundant proteins and lipids near the surface. In addition, we compared transcriptomes of A. oryzae in the different types of rice koji. Finally, we found that A. oryzae increases the nuclear number and hyphal width in the course of 1-3 days cultivation. Our imaging analyses indicate that A. oryzae hyphae grew more deeply into 50% polished rice than 90% polished rice. The increases of nuclear number may be a selectively acquired characteristic for the high secretory capacity during the long history of cultiva...Continue Reading

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Citations

Mar 10, 2021·Bioscience, Biotechnology, and Biochemistry·Yujiro Higuchi
Mar 18, 2021·MBio·Sayumi FukudaNorio Takeshita
Jun 9, 2021·Journal of Microbiology and Biotechnology·Su Bin Ji, Chae Hun Ra

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Datasets Mentioned

BETA
DRA009542

Methods Mentioned

BETA
confocal reflection microscopy
X-ray
RNA-seq
fluorescence microscopy
confocal microscopy

Software Mentioned

CLC Genomic Workbench
ZEN
ImageJ
VG Studio MAX

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