Invasive hyphal growth: an F-actin depleted zone is associated with invasive hyphae of the oomycetes Achlya bisexualis and Phytophthora cinnamomi

Fungal Genetics and Biology : FG & B
Sophie K WalkerAshley Garrill

Abstract

We have compared F-actin patterns in invasive and non-invasive oomycete hyphae. In Achlya bisexualis an F-actin depleted zone is present in 70% of invasive but only 9% of non-invasive hyphae. In Phytophthora cinnamomi these figures are 74 and 20%, respectively. Thus, the F-actin depleted zone appears to be associated with invasive growth. TEM images indicate that it is unlikely to represent areas of vesicle accumulation. Measurements of turgor indicate no significant increase under invasive conditions (0.65 MPa (invasive) and 0.63 MPa (non-invasive)). Similarly we found no difference in burst pressures (1.04 MPa (invasive) and 1.06 MPa (non-invasive)), although surrounding agarose may lead to overestimates of invasive tip strength. An F-actin depleted zone has the potential, along with wall softening, to increase protrusive force in the absence of turgor increases. Staining of F-actin in hyphae under hyperosmotic conditions suggests that decreases in F-actin at growing tips may also enable non-invasive growth at very low turgor.

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Citations

Sep 10, 2013·Journal of Experimental Botany·Amir Sanati Nezhad, Anja Geitmann
Oct 6, 2012·Fungal Genetics and Biology : FG & B·Tijs KetelaarFrancine Govers
Jul 7, 2009·Trends in Microbiology·Trudy Torto-AlaliboRalph A Dean
Nov 4, 2006·Cellular Microbiology·Adrienne R Hardham
Dec 19, 2013·Cellular Microbiology·Harold J G MeijerFrancine Govers
Nov 27, 2015·Microbiology·Abishek MuralidharAshley Garrill
Oct 8, 2016·Cellular and Molecular Life Sciences : CMLS·Kiki KotsTijs Ketelaar
Aug 30, 2018·The New Phytologist·Roger R Lew
Jun 7, 2011·Nature Reviews. Microbiology·Roger R Lew
Jul 6, 2014·Lab on a Chip·A Sanati Nezhad
Jun 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Marie HeldDan V Nicolau
May 26, 2021·Current Biology : CB·Youssef ChebliAnja Geitmann

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