Invasive potential of bacterial isolates associated with subclinical bovine mastitis

Research in Veterinary Science
José L Anaya-LópezAlejandra Ochoa-Zarzosa

Abstract

This work describes differences in the invasive ability of bacterial isolates associated with mastitis. Invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (BMEC). BMEC were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mRNAs. Ten bacterial isolates comprising the staphylococcal species Staphylococcus aureus (3), Staphylococcus epidermidis (1), Staphylococcus haemolyticus (1), Staphylococcus equorum (2), Staphylococcus xylosus (1) and Brevibacterium stationis (2) obtained from raw milk of cows with mastitis from backyard farms were assayed for their ability to invade BMEC. Only two S. aureus and one S. epidermidis isolates were able to invade BMEC, at similar levels to the S. aureus control strain ATCC 27543. In conclusion, using the in vitro model of infection used in this study, differences in bacterial invasion capability may be detected.

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Citations

Jul 8, 2009·Research in Veterinary Science·Reza Ghanbarpour, Eric Oswald
Feb 3, 2009·Research in Veterinary Science·Joel E López-MezaAlejandra Ochoa-Zarzosa
Aug 3, 2006·The Journal of Infection·Javier Oviedo-BoysoVíctor M Baizabal-Aguirre
Aug 17, 2011·Microbial Pathogenesis·Leticia Lara-ZárateAlejandra Ochoa-Zarzosa
Feb 5, 2009·FEMS Microbiology Letters·Javier Oviedo-BoysoVíctor M Baizabal-Aguirre
May 11, 2019·Tropical Animal Health and Production·Ronaldo M BianchiDavid Driemeier

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