PMID: 6443256Jan 1, 1983

Invasiveness and tumorigenicity of MO4 mouse fibrosarcoma cells pretreated with microtubule inhibitors

Clinical & Experimental Metastasis
C MeyvischM M Mareel

Abstract

MO4 cell aggregates with a diameter of 0.3 mm produced invasive fibrosarcomas after s.c. implantation into the pinna of syngeneic mice. Histology of pinnae fixed 10 min to 5 days after implantation of an aggregate suggested that the tumour was produced by the cells that invaded during the first day, and that the cells remaining in the aggregate were eliminated by the reaction of the host. Before implantation we have pretreated MO4 cell aggregates with 1 microgram/ml of the microtubule inhibitors Nocodazole (ND) and vincristine (VCR), known to inhibit both proliferation and invasion, and with 1 microgram/ml 5-fluorouracil (5-FU), known to inhibit proliferation but not invasion. Tumorigenicity was significantly reduced after treatment with ND or VCR, as compared to treatment with 5-FU or to controls. Histology of pinnae fixed 10 min to 3 days after implantation showed absence or scarceness of invasive MO4 cells after pretreatment with ND or VCR, in contrast with controls or with aggregates pretreated with 5-FU. The effect of ND, VCR and 5-FU on the growth of aggregates in culture on gyrotory shaker was reversible within 1 and 2 days respectively. After treatment with ND or VCR slight alterations in the function of the cytoplasmic...Continue Reading

Citations

Jan 1, 1983·Cancer Metastasis Reviews·C Meyvisch
Jan 1, 1986·Cancer Metastasis Reviews·R G Greig, D L Trainer
Oct 27, 2007·Cancer Immunology, Immunotherapy : CII·Paula ChiarellaRaúl A Ruggiero
Feb 12, 1998·The Clinical Journal of Pain·B S GalerJ A Turner
Jul 1, 1990·Cancer Metastasis Reviews·M M MareelP De Baetselier
May 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L MessiaenM M Mareel

Related Concepts

Metazoa
Antineoplastic Agents
Benzimidazoles
Cell Aggregation
Cell Division Phases
External Ear
Fibrosarcoma
5-FU medac
Mice, Inbred C3H
Mitomycins

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