Inventory of fatty acid desaturases in the pennate diatom Phaeodactylum tricornutum

Marine Drugs
Lina-Juana Dolch, Eric Maréchal

Abstract

The diatom Phaeodactylum is rich in very long chain polyunsaturated fatty acids (PUFAs). Fatty acid (FA) synthesis, elongation, and desaturation have been studied in depth in plants including Arabidopsis, but for secondary endosymbionts the full picture remains unclear. FAs are synthesized up to a chain length of 18 carbons inside chloroplasts, where they can be incorporated into glycerolipids. They are also exported to the ER for phospho- and betaine lipid syntheses. Elongation of FAs up to 22 carbons occurs in the ER. PUFAs can be reimported into plastids to serve as precursors for glycerolipids. In both organelles, FA desaturases are present, introducing double bonds between carbon atoms and giving rise to a variety of molecular species. In addition to the four desaturases characterized in Phaeodactylum (FAD2, FAD6, PtD5, PtD6), we identified eight putative desaturase genes. Combining subcellular localization predictions and comparisons with desaturases from other organisms like Arabidopsis, we propose a scheme at the whole cell level, including features that are likely specific to secondary endosymbionts.

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Citations

Oct 16, 2015·Scientific Reports·Coline MeïFabrice Rébeillé
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Weichao Huang, Fayza Daboussi
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Olga SayanovaJohnathan A Napier
Sep 24, 2020·Marine Drugs·Alfonsina MilitoAnna Palumbo
Feb 27, 2020·Biology·Ben LeylandInna Khozin-Goldberg
Jul 11, 2019·Biochimie·Eric Maréchal, Josselin Lupette
Mar 7, 2020·Environmental Pollution·Carmen González-FernándezPhilippe Soudant
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Apr 2, 2021·Plant Physiology·Elodie BilleyEric Maréchal
Sep 10, 2019·Progress in Lipid Research·Christian MorabitoFabrice Rébeillé
May 12, 2020·Biochimie·Josselin Lupette, Christoph Benning
May 11, 2021·Frontiers in Plant Science·Nolwenn GuéguenEric Maréchal

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Methods Mentioned

BETA
acylation

Software Mentioned

BLASTP
HECTAR
ASAFind

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