Inventory of the GH70 enzymes encoded by Leuconostoc citreum NRRL B-1299 - identification of three novel α-transglucosylases

The FEBS Journal
Delphine PasseriniClaire Moulis

Abstract

Leuconostoc citreum NRRL B-1299 has long been known to produce α-glucans containing both α-(1→6) and α-(1→2) linkages, which are synthesized by α-transglucosylases of the GH70 family. We sequenced the genome of Leuconostoc citreum NRRL B-1299 to identify the full inventory of GH70 enzymes in this strain. Three new genes (brsA, dsrM and dsrDP) putatively encoding GH70 enzymes were identified. The corresponding recombinant enzymes were characterized. Branching sucrase A (BRS-A) grafts linear α-(1→6) dextran with α-(1→2)-linked glucosyl units, and is probably involved in the α-(1→2) branching of L. citreum NRRL B-1299 dextran. This is the first report of a naturally occurring α-(1→2) branching sucrase. DSR-M and DSR-DP are dextransucrases that are specific for α-(1→6) linkage synthesis and mainly produce oligomers or short dextrans with molar masses between 580 and 27 000 g·mol(-1) . In addition, DSR-DP contains sequences that diverge from the consensus sequences that are typically present in enzymes that synthesize linear dextran. Comparison of the genome with five other L. citreum genomes further revealed that dsrDP is unique to L. citreum NRRL B-1299. The presence of this gene in a prophage represents the first evidence of phag...Continue Reading

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Citations

Oct 7, 2015·Frontiers in Microbiology·María I TorinoFernanda Mozzi
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Claire MoulisMagali Remaud-Simeon
May 8, 2016·Cellular and Molecular Life Sciences : CMLS·Štefan Janeček, Marek Gabriško
Jan 15, 2016·The Journal of Biological Chemistry·Marlène VuilleminMagali Remaud-Siméon
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May 28, 2019·Journal of Agricultural and Food Chemistry·Franziska MünkelDaniel Wefers
Dec 16, 2021·Critical Reviews in Food Science and Nutrition·Xiaodan LiWeifeng Liu

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