PMID: 9425602Jan 13, 1998Paper

Inverse relationship between membrane lipid fluidity and activity of Na+/H+ exchangers, NHE1 and NHE3, in transfected fibroblasts

The Journal of Membrane Biology
C BooksteinE B Chang

Abstract

This report presents a study of the effects of the membrane fluidizer, benzyl alcohol, on NHE isoforms 1 and 3. Using transfectants of an NHE-deficient fibroblast, we analyzed each isoform separately. An increase in membrane fluidity resulted in a decrease of approximately 50% in the specific activities of both NHE1 and NHE3. Only Vmax was affected; KNa was unchanged. This effect was specific, as Na+, K+, ATPase activity was slightly stimulated. Inhibition of NHE1 and NHE3 was reversible and de novo protein synthesis was not required to restore NHE activity after washout of fluidizer. Inhibition kinetics of NHE1 by amiloride, 5-(N,N-dimethyl)amiloride (DMA), 5-(N-hexamethyl)amiloride (HMA) and 5-(N-ethyl-N-isopropyl)amiloride (EIPA) were largely unchanged. Half-maximal inhibition of NHE3 was also reached at approximately the same concentrations of amiloride and analogues in control and benzyl alcohol treated, suggesting that the amiloride binding site was unaffected. Inhibition of vesicular transport by incubation at 4 degrees C augmented the benzyl alcohol inhibition of NHE activity, suggesting that the fluidizer effect does not solely involve vesicle trafficking. In summary, our data demonstrate that the physical state of mem...Continue Reading

Citations

Nov 5, 2011·The Journal of Membrane Biology·Val Andrew FajardoPaul J LeBlanc
Nov 20, 2013·Chemico-biological Interactions·Béatrice DendeléDominique Lagadic-Gossmann
Aug 17, 2005·European Journal of Clinical Investigation·S C NarinsM Z Abedin
Dec 18, 2013·Biochemical and Biophysical Research Communications·Pawared OntongNaoki Itano
Dec 27, 2016·Journal of Cellular Physiology·Anja Paehler Vor der NolteUrsula Seidler
Feb 12, 2008·Journal of Cellular Physiology·Xavier TekpliDominique Lagadic-Gossmann
Feb 7, 2001·American Journal of Physiology. Cell Physiology·P R KielaF K Ghishan

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