Inverse tissue mechanics of cell monolayer expansion

PLoS Computational Biology
Yohei KondoShin Ishii

Abstract

Living tissues undergo deformation during morphogenesis. In this process, cells generate mechanical forces that drive the coordinated cell motion and shape changes. Recent advances in experimental and theoretical techniques have enabled in situ measurement of the mechanical forces, but the characterization of mechanical properties that determine how these forces quantitatively affect tissue deformation remains challenging, and this represents a major obstacle for the complete understanding of morphogenesis. Here, we proposed a non-invasive reverse-engineering approach for the estimation of the mechanical properties, by combining tissue mechanics modeling and statistical machine learning. Our strategy is to model the tissue as a continuum mechanical system and to use passive observations of spontaneous tissue deformation and force fields to statistically estimate the model parameters. This method was applied to the analysis of the collective migration of Madin-Darby canine kidney cells, and the tissue flow and force were simultaneously observed by the phase contrast imaging and traction force microscopy. We found that our monolayer elastic model, whose elastic moduli were reverse-engineered, enabled a long-term forecast of the t...Continue Reading

References

Feb 19, 2002·Trends in Cell Biology·Karen A Beningo, Yu-Li Wang
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lars HufnagelBoris I Shraiman
Jul 24, 2007·Nature Reviews. Molecular Cell Biology·Thomas Lecuit, Pierre-François Lenne
Oct 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·M PoujadeP Silberzan
Jan 17, 2008·Optics Letters·Manuel Guizar-SicairosJames R Fienup
Jan 31, 2008·Pflügers Archiv : European journal of physiology·Jan Christian Martens, Manfred Radmacher
Apr 7, 2009·Bioinformatics·Stephan PreibischPavel Tomancak
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Philippe MarmottantHélène Delanoë-Ayari
May 6, 2010·Biophysical Journal·L PetitjeanP Silberzan
Aug 18, 2010·IEEE Transactions on Pattern Analysis and Machine Intelligence·Thomas Brox, Jitendra Malik
Sep 28, 2010·Physical Review Letters·Karine GuevorkianFrançoise Brochard-Wyart
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonas RanftFrank Jülicher
May 3, 2011·Journal of the Mechanics and Physics of Solids·D AmbrosiK Garikipati
May 24, 2011·Nature Materials·Dhananjay T TambeXavier Trepat
May 26, 2012·Journal of the Royal Society, Interface·Isabelle BonnetFrançois Graner
Jul 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sri Ram Krishna VedulaBenoît Ladoux
Sep 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrew R HarrisGuillaume T Charras
May 28, 2013·Cell·Carl-Philipp Heisenberg, Yohanns Bellaïche
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Martine Ben Amar, Fei Jia
Jun 25, 2013·Nature Materials·Jae Hun KimJeffrey J Fredberg
Sep 21, 2013·Development·Kaoru Sugimura, Shuji Ishihara
Dec 10, 2013·Nature Methods·Otger CampàsDonald E Ingber
Jan 15, 2014·Biophysical Journal·Olivier Cochet-EscartinPhilippe Marcq
Jul 26, 2014·Physical Review Letters·Rhoda J Hawkins, Tanniemola B Liverpool
May 10, 2015·The European Physical Journal. E, Soft Matter·Sham TliliPierre Saramito
Dec 15, 2015·ELife·Boris GuiraoYohanns Bellaïche
Dec 27, 2015·Physical Review Letters·Romaric VincentXavier Trepat
Jan 21, 2016·Development·Kaoru SugimuraFrançois Graner
Jan 23, 2016·Soft Matter·Pierre RechoPhilippe Marcq
Apr 14, 2016·Biophysical Journal·Vincent NierPhilippe Marcq

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Methods Mentioned

BETA
force measurement
force

Software Mentioned

Fiji

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