Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases

FEBS Letters
P BielyJ Visser

Abstract

Endopolygalacturonases I and II (PGI and PGII) of Aspergillus niger and an exopolygalacturonase (ExoPG) of A. tubingensis were investigated to reveal the stereochemistry of their hydrolytic action. Reduced pentagalacturonic acid (pentaGalU-ol) and reduced trigalacturonic acid (triGalU-ol) were used as non-reducing substrates for the enzymes. The configuration of the reducing ends in the products formed in D2O reaction mixtures was followed by 1H-NMR spectroscopy. It has been unambiguously established that primary cleavage of pentaGalU-ol by both PGI and PGII leads to diGalU-ol and the beta-anomer of triGalUA. The primary products of hydrolysis of triGalUA-ol by ExoPG were diGal-ol and the beta-anomer of GalUA. Thus, all three Aspergillus polygalacturonases belong to the so-called inverting glycanases, i.e. they utilize the single displacement mechanism of hydrolysis of the glycosidic linkage.

References

Jan 1, 1976·Advances in Carbohydrate Chemistry and Biochemistry·L Rexová-Benková, O Markovic
Mar 26, 1993·Biochimica Et Biophysica Acta·P BielyF Shareck
Sep 15, 1996·European Journal of Biochemistry·H C KesterJ Visser

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Citations

Dec 19, 2001·Progress in Biophysics and Molecular Biology·J Jenkins, R Pickersgill
Apr 26, 2000·Biochimica Et Biophysica Acta·P BielyJ Visser
Jun 19, 2001·Biochimica Et Biophysica Acta·E BonninJ Thibault
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Jan 14, 2009·The European Physical Journal. E, Soft Matter·R R VincentM A K Williams
Mar 5, 2004·Proteins·Jong Keun ChoiWhanchul Shin
May 16, 2006·Analytical Biochemistry·Bryan WoosleyCarl Bergmann
Jan 21, 2011·Plant Signaling & Behavior·Steve SwainMikihiro Ogawa
Sep 2, 1998·Journal of Structural Biology·J JenkinsR Pickersgill
Sep 20, 2005·Journal of Biotechnology·Barbara G Klug-SantnerGeorg M Guebitz
May 27, 2020·Nature Microbiology·Andreas SichertJan-Hendrik Hehemann
Sep 12, 1998·The Journal of Biological Chemistry·R PickersgillJ Jenkins
Mar 27, 1999·European Journal of Biochemistry·J A BenenJ Visser
Oct 18, 2005·Carbohydrate Research·Mark R StamBernard Henrissat

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