PMID: 7012837Dec 1, 1980Paper

Inversions of specific DNA segments in flagellar phase variation of Salmonella and inversion systems of bacteriophages P1 and Mu

Proceedings of the National Academy of Sciences of the United States of America
K Kutsukake, T Iino

Abstract

Prophages P1 and Mu produces a trans-acting factor possessing the din+ activity which catalyzes the inversion of the specific DNA segment responsible for flagellar phase variation of Salmonella, din mutants were isolated from PICMclr100 phage by selecting phages that did not suppress the yh2 mutation of Salmonella in prophage state. No inversion loop structure was detected among DNA forms arising after denaturation and rehybridization of DNAs extracted from the din mutants. The DNA fragment containing C region of P1 was cloned on a plasmid vector, pCR1. The resulting hybrid plasmid, pKK2, was shown to possess the din+ activity: the vh2 mutant of Salmonella harboring the plasmid changed the flagellar phase. From analysis of the plasmid by use of BamHI and Bgl II, the din gene specifying the din+ activity was located near or within the C region of P1. It is highly plausible that the din gene of P1 was also involved in the inversion of the C region. Similarly, the DNA fragment containing the G and beta segments of Mu was cloned on pCR1. The resulting hybrid plasmid, pII101, also possessed the din+ activity.

References

Apr 8, 1977·Science·J ZiegM Simon
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M SilvermanM Simon
May 7, 1976·Molecular & General Genetics : MGG·C CoveyJ Carbon
Oct 1, 1976·Virology·L T Chow, A I Bukhari
Mar 15, 1975·Journal of Molecular Biology·B Allet, A I Bukhari
Nov 14, 1975·Science·M M Howe, E G Bade
Jan 1, 1975·Virology·A I Bukhari, B Allet
May 1, 1973·Journal of General Microbiology·H FujitaT Iino
Nov 25, 1973·Journal of Molecular Biology·H Suzuki, T Iino
Nov 1, 1967·Journal of General Microbiology·U B Pearce, B A Stocker

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·S HarayamaT Iino
Mar 26, 1986·Biochimica Et Biophysica Acta·R KanaarN R Cozzarelli
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R H PlasterkP van de Putte
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R H PlasterkP van de Putte
May 31, 2003·Journal of Bacteriology·Heather R Bonifield, Kelly T Hughes
Nov 18, 2004·Journal of Bacteriology·Jeremy S WebbStaffan Kjelleberg
Jan 1, 1983·Molecular & General Genetics : MGG·K E KennedyW Arber
Sep 1, 1982·Plasmid·F SchmidtB Wiedemann
Jun 30, 2004·Journal of Molecular Biology·Erin R Sanders, Reid C Johnson
Apr 6, 2018·PLoS Genetics·Ognjen SekulovicAndrew Camilli
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Jun 1, 1984·Journal of Bacteriology·F E Nano, S Kaplan
Nov 1, 1984·Molecular and Cellular Biology·J Rubnitz, S Subramani
Jun 25, 2015·Microbiology Spectrum·Reid C Johnson
Jan 1, 1986·Journal of Bacteriology·T KomanoT Nisioka
Jul 1, 1983·Journal of Bacteriology·E Szekely, M Simon
Oct 5, 2021·Molecular Microbiology·Babita Adhikari Dhungel, Revathi Govind

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