Invertebrate cell culture considerations: insects, ticks, shellfish, and worms

Methods in Cell Biology
C J Bayne

Abstract

Establishment of cell lines from insect and arachnid invertebrates has become routine, whereas other invertebrate taxa have been frustratingly unproductive of cell lines. None is available for any marine invertebrate, despite a strong and well-recognized need for cell lines from species that are important in aquaculture, from parasite vectors and intermediate hosts of parasites, from parasites themselves, from certain biomedical models, and from other species that are pests. Drawing on experiences gained attempting to establish cell lines from molluscs and trematodes and on published and ongoing research with diverse invertebrates, this chapter attempts to anticipate the problems that are likely to be encountered in such endeavors and discusses possible solutions. Criteria to be considered in the selection of basic culture media, temperature, pH, and media additives; approaches that have been developed to yield sterile primary cultures; and factors to consider in decisions about feeding schedules, retention of tissue fragments and nonadherent cells, use of heterologous feeder layers, and other variables are described. Suggestions are made concerning means to objectively score the success of tested variables and means to induce ...Continue Reading

Citations

Sep 1, 2005·Marine Biotechnology·Baruch Rinkevich
Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Apr 19, 2018·Frontiers in Physiology·Valeria MaselliAnna Di Cosmo
Jul 17, 2014·Cell and Tissue Research·Cristiano Di BenedettoMaria Daniela Candia Carnevali

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