Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.
Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
Lineage mapping the pre-implantation mouse embryo by two-photon microscopy, new insights into the segregation of cell fates.
A new Canterbury tale: the eighth International Meeting on Yeast Apoptosis in Canterbury, UK, 2-6 May 2011.
Inverted selective plane illumination microscopy (iSPIM) enables coupled cell identity lineaging and neurodevelopmental imaging in Caenorhabditis elegans.
Live imaging of whole mouse embryos during gastrulation: migration analyses of epiblast and mesodermal cells
Limited predictive value of blastomere angle of division in trophectoderm and inner cell mass specification
Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages
Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
Automated C. elegans embryo alignments reveal brain neuropil position invariance despite lax cell body placement
Control of the proportion of inner cells by asymmetric divisions and the ensuing resilience of cloned rabbit embryos
Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos
Light sheet microscopy with high spatial resolution based on polarized structured illumination beam modulated by the phase mask
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
Mysteries in embryonic development: How can errors arise so frequently at the beginning of mammalian life?
Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops
Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture
Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging
Supervised learning to quantify amyloidosis in whole brains of an Alzheimer's disease mouse model acquired with optical projection tomography
Non-destructive 3D Microtomography of Cerebral Angioarchitecture Changes Following Ischemic Stroke in Rats Using Synchrotron Radiation
Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited
Performance tradeoffs for single- and dual-objective open-top light-sheet microscope designs: a simulation-based analysis.
Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches.
Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.
Cell Fate Specification
Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.