Inverted light-sheet microscope for imaging mouse pre-implantation development

Nature Methods
Petr StrnadJan Ellenberg

Abstract

Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.

References

Aug 7, 2004·Developmental Cell·Janet Rossant, Patrick P L Tam
Jan 7, 2005·Development·Karolina Piotrowska-NitscheMagdalena Zernicka-Goetz
May 10, 2005·Genes & Development·Nami MotosugiTakashi Hiiragi
Dec 13, 2005·Nature Reviews. Molecular Cell Biology·Magdalena Zernicka-Goetz
Aug 8, 2006·The International Journal of Developmental Biology·Takashi HiiragiDavor Solter
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Nov 6, 2007·Development·Jens-Erik Dietrich, Takashi Hiiragi
Feb 28, 2008·Cells, Tissues, Organs·Jens Erik Dietrich, Takashi Hiiragi
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samantha A MorrisMagdalena Zernicka-Goetz
Aug 10, 2010·Reproductive Biomedicine Online·P L Wale, D K Gardner
Oct 27, 2010·IEEE Transactions on Pattern Analysis and Machine Intelligence·Andriy Myronenko, Xubo Song
Jan 25, 2011·Nature Cell Biology·Nicolas PlachtaPeriklis Pantazis
Mar 30, 2011·Genesis : the Journal of Genetics and Development·Takaya AbeToshihiko Fujimori
Aug 9, 2011·Nature Biotechnology·Jérémie CapouladeMichael Knop
Oct 4, 2011·Current Opinion in Genetics & Development·Michael Weber, Jan Huisken
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yicong WuHari Shroff
Jun 5, 2012·Nature Methods·Uros KrzicLars Hufnagel
Nov 28, 2012·Current Biology : CB·Inna TabanskyKevin Eggan
Jan 21, 2014·Current Topics in Developmental Biology·Eszter PosfaiJanet Rossant
Mar 11, 2015·Cell Reports·Panagiotis XenopoulosAnna-Katerina Hadjantonakis

❮ Previous
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Citations

Dec 30, 2016·Current Opinion in Cell Biology·Melanie D WhiteNicolas Plachta
Mar 23, 2017·Nature·Young Seok JuMichael R Stratton
Dec 8, 2016·Nature Biotechnology·Lars Hufnagel, Rainer Pepperkok
Apr 1, 2017·Nature Methods·Rory M Power, Jan Huisken
Jul 1, 2017·Nature Methods·P Philippe LaissueHari Shroff
Jul 28, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jaroslav IchaCaren Norden
Aug 10, 2017·IEEE Transactions on Cybernetics·Lifei BaiHuijun Gao
Dec 14, 2017·Biologie aujourd'hui·Jean-Léon Maître
Aug 11, 2018·Annual Review of Cell and Developmental Biology·Hui Ting Zhang, Takashi Hiiragi
Nov 6, 2018·The FEBS Journal·Aline Xavier da Silveira Dos Santos, Prisca Liberali
Jul 14, 2018·Science·Agata P Zielinska, Melina Schuh
Jun 18, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kamila CzechowskaAttila Tárnok
Apr 17, 2020·Journal of Cell Science·Björn EismannChristian Conrad
Sep 7, 2018·Nature Plants·Miroslav OvečkaJozef Šamaj
Feb 20, 2019·Journal of Assisted Reproduction and Genetics·David F Albertini
Jul 6, 2019·Nature Communications·Adam K GlaserJonathan T C Liu
Jul 7, 2017·Biology of the Cell·Jean-Léon Maître
Mar 25, 2020·Human Reproduction Open·UNKNOWN ESHRE Working group on Time-lapse technologyGiovanni Coticchio
Jul 19, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Jonathan R Chubb
Oct 18, 2019·Nature Methods·Rory M Power, Jan Huisken
Apr 14, 2018·PLoS Computational Biology·Martin WeigertMoritz Kreysing
May 12, 2018·Nature Biomedical Engineering·Adam K GlaserJonathan T C Liu
Jul 25, 2018·Nature Cell Biology·Marta N Shahbazi, Magdalena Zernicka-Goetz
Jul 13, 2019·Annual Review of Cell and Developmental Biology·Yinan WanPhilipp J Keller
Sep 2, 2020·Annual Review of Genetics·Jonathan FiorentinoAntonio Scialdone
Jan 10, 2021·Nature Cell Biology·Eszter PosfaiJanet Rossant
Dec 8, 2020·Biomedical Optics Express·Lindsey A BarnerJonathan T C Liu
Feb 17, 2021·Nature Biomedical Engineering·Jonathan T C LiuAnant Madabhushi

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