Investigating filamentous growth and biofilm/mat formation in budding yeast

Cold Spring Harbor Protocols
Paul J Cullen

Abstract

In response to nutrient limitation, budding yeast can undergo filamentous growth by differentiating into elongated chains of interconnected cells. Filamentous growth is regulated by signal transduction pathways that oversee the reorganization of cell polarity, changes to the cell cycle, and an increase in cell adhesion that occur in response to nutrient limitation. Each of these changes can be easily measured. Yeast can also grow colonially atop surfaces in a biofilm or mat of connected cells. Filamentous growth and biofilm/mat formation require cooperation among individuals; therefore, studying these responses can shed light on the origin and genetic basis of multicellular behaviors. The assays introduced here can be used to study analogous behaviors in other fungal species, including pathogens, which require filamentous growth and biofilm/mat formation for virulence.

References

May 1, 1995·The Journal of Cell Biology·J Chant, J R Pringle
Sep 1, 1994·Molecular Biology of the Cell·S J KronG R Fink
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M G LambrechtsI S Pretorius
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·B GuoG R Fink
Feb 7, 2001·Science·T B Reynolds, G R Fink
Aug 22, 2001·Molecular Biology of the Cell·H A HarkinsJ R Pringle
Sep 11, 2002·Molecular Biology of the Cell·Paul J Cullen, George F Sprague
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Hay-Oak Park, Erfei Bi
Jan 29, 2010·PLoS Genetics·Joshua A Granek, Paul M Magwene
Apr 24, 2010·Science·Robin D DowellCharles Boone
Jul 14, 2010·Current Biology : CB·Sheelarani KarunanithiPaul J Cullen
Aug 31, 2011·The Journal of Cell Biology·Libuse VáchováZdena Palková
Jan 6, 2012·Genetics·Paul J Cullen, George F Sprague
Sep 18, 2012·Science·Owen RyanCharles Boone
Mar 4, 2015·Cold Spring Harbor Protocols·Paul J Cullen

❮ Previous
Next ❯

Citations

Sep 29, 2017·Journal of the Royal Society, Interface·Hayden TronnoloneBenjamin J Binder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.