Investigating intestinal inflammation in DSS-induced model of IBD.

Journal of Visualized Experiments : JoVE
Janice J KimWaliul I Khan

Abstract

Inflammatory bowel disease (IBD) encompasses a range of intestinal pathologies, the most common of which are ulcerative colitis (UC) and Crohn's Disease (CD). Both UC and CD, when present in the colon, generate a similar symptom profile which can include diarrhea, rectal bleeding, abdominal pain, and weight loss.(1) Although the pathogenesis of IBD remains unknown, it is described as a multifactorial disease that involves both genetic and environmental components.(2) There are numerous and variable animal models of colonic inflammation that resemble several features of IBD. Animal models of colitis range from those arising spontaneously in susceptible strains of certain species to those requiring administration of specific concentrations of colitis-inducing chemicals, such as dextran sulphate sodium (DSS). Chemical-induced models of gut inflammation are the most commonly used and best described models of IBD. Administration of DSS in drinking water produces acute or chronic colitis depending on the administration protocol.(3) Animals given DSS exhibit weight loss and signs of loose stool or diarrhea, sometimes with evidence of rectal bleeding.(4,5) Here, we describe the methods by which colitis development and the resulting ...Continue Reading

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Citations

Oct 12, 2012·Journal of Visualized Experiments : JoVE·Hon Wai KoonCharalabos Pothoulakis
Jan 19, 2016·Life Sciences·Mohamed BalahaWalaa Elwan
Nov 3, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Fengmin ShiGencheng Han
Dec 25, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Annika FredeAstrid M Westendorf
Sep 6, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Brandon M ZeiglerBjorn Thorsrud
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yang YangYongmin Zhang
Jul 13, 2016·Mediators of Inflammation·Patrícia Reis de SouzaCristina Ribeiro de Barros Cardoso
Jun 28, 2016·Biochemical Pharmacology·Marina de Paula-SilvaSonia Maria Oliani
Jun 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Babajide O AjayiEbenezer O Farombi
Mar 25, 2015·PLoS Neglected Tropical Diseases·Chinyere K OkoroRobert A Kingsley
Oct 14, 2016·Molecular Diversity·María Gálvez-LlompartJorge Gálvez
Mar 25, 2017·Nutrients·Federica CioffiSusanna Iossa
Sep 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hana Sarashina-KidaTadatsugu Taniguchi
Dec 21, 2017·Frontiers in Cellular and Infection Microbiology·Ramanathan SrinivasanArumugam Veera Ravi
Mar 22, 2018·American Journal of Physiology. Cell Physiology·Trey S RottgenVazhaikkurichi M Rajendran
Feb 7, 2018·Molecular Neurobiology·Pablo Garrido-GilJose L Labandeira-Garcia
Jan 9, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kandy T VelázquezE Angela Murphy
Jun 13, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Vladimir GrubišićBrian D Gulbransen
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Oct 9, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ewa ŻyłaJoanna Gromadzka-Ostrowska
Apr 14, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·X L WangM Qiao
Jan 9, 2020·American Journal of Physiology. Cell Physiology·Kumar KotloBrian T Layden
Feb 1, 2020·The FEBS Journal·Ibrahim M SayedSoumita Das

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