Investigating Possible Enzymatic Degradation on Polymer Shells around Inorganic Nanoparticles

International Journal of Molecular Sciences
Lin ZhuWolfgang J Parak

Abstract

Inorganic iron oxide nanoparticle cores as model systems for inorganic nanoparticles were coated with shells of amphiphilic polymers, to which organic fluorophores were linked with different conjugation chemistries, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry and two types of "click chemistry". The nanoparticle-dye conjugates were exposed to different enzymes/enzyme mixtures in order to investigate potential enzymatic degradation of the fluorophore-modified polymer shell. The release of the dyes and polymer fragments upon enzymatic digestion was quantified by using fluorescence spectroscopy. The data indicate that enzymatic cleavage of the fluorophore-modified organic surface coating around the inorganic nanoparticles in fact depends on the used conjugation chemistry, together with the types of enzymes to which the nanoparticle-dye conjugates are exposed.

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Citations

May 16, 2021·Journal of Hazardous Materials·Renata Aparecida MonteiroLeonardo Fernandes Fraceto
Oct 18, 2019·Bioconjugate Chemistry·Sathi RoyWolfgang J Parak

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Methods Mentioned

BETA
PMA
electrophoresis
dynamic light scattering

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