Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Sebastian MuellerKai Januschowski

Abstract

Retidyne™ is a new lutein-based dye for internal limiting membrane staining. It uses the intrinsic staining characteristics of lutein which is already known to act as an antioxidant and blue-light filter in the human retina. We investigated retinal tolerance to different staining times measured by the electroretinogram (ERG) of an isolated and perfused retina whole mount. For functionality, testing bovine retinas were prepared and perfused with an oxygen saturated standard solution and the ERG was recorded until stable b-wave amplitudes were reached. Then the perfusion was stopped and Retidyne™ was applied directly onto the retinal surface for exposure times of 60 or 120 s. After restarting the perfusion with standard solution, the ERG amplitudes were monitored for 75 min. To investigate the effects on photoreceptor function alone, 1 mM asparate was added to block b-waves. For an exposure time of 60 s amplitudes of a- and b-waves remained stable throughout the experiment. Exposure times of 120 s caused an initial drop of amplitudes that reached statistical significance only for a-waves (a, - 21%, p = 0.047; b, - 14%, p = 0.052). This effect was only seen during the first minutes of the washout and the ERG recovered completely. ...Continue Reading

References

Jan 1, 1985·Vision Research·R A BoneS L Tarsis
Aug 2, 2000·Archives of Ophthalmology·K KadonosonoS Ohno
Nov 25, 2000·The British Journal of Ophthalmology·P R van den BiesenD van Norren
Jul 7, 2001·Free Radical Biology & Medicine·S P Sundelin, S E Nilsson
Apr 6, 2002·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Hiroshi EnaidaTatsuro Ishibashi
Jul 26, 2002·American Journal of Ophthalmology·Peter StalmansIngeborg Stalmans
Jan 23, 2003·International Journal of Toxicology·Randolph D Glickman
May 9, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·S WolfU E K Schnurrbusch
Oct 20, 2004·Retina·Brendan J VoteBrian C Joondeph
Feb 19, 2005·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·San-Ni ChengCheng-Kuo Cheng
Nov 9, 2005·Brain Research. Brain Research Protocols·Matthias LükeToni Schneider
Feb 28, 2008·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Eduardo B Rodrigues, Carsten H Meyer
Jun 7, 2008·Developments in Ophthalmology·Gregor Wollensak
Jan 14, 2009·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·S ThalerC Haritoglou
May 13, 2009·Acta Ophthalmologica·Paul B HenrichSiegfried Priglinger
Nov 26, 2009·Retina·Burkhard von JagowChristos Haritoglou
Apr 12, 2011·Klinische Monatsblätter für Augenheilkunde·A HöingC Haritoglou
Apr 3, 2012·Progress in Retinal and Eye Research·Aize KijlstraTos T J M Berendschot
Jul 28, 2013·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Julia LükeMatthias Lüke
Jan 21, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Emmerson BadaroRubens Belfort
Jun 6, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Bruno Albuquerque FurlaniRubens Belfort
Aug 26, 2014·Current Eye Research·Ricardo P Casaroli-MaranoMaurício Maia
Sep 14, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Aysegül TuraUNKNOWN International Chromovitrectomy Collaboration
Apr 27, 2016·Klinische Monatsblätter für Augenheilkunde·H Gerding
Jan 14, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Heinrich Gerding
Mar 31, 2017·Current Eye Research·José HurstKai Januschowski

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Citations

Jul 30, 2020·Progress in Retinal and Eye Research·Sven SchnichelsAlexa Klettner

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