Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry.

Current Protocols in Protein Science
Christopher R Morgan, John R Engen

Abstract

By taking advantage of labeling methods such as hydrogen exchange (HX), many details about protein conformation, dynamics, and interactions can be revealed by mass spectrometry. In this unit, hydrogen exchange theory is discussed as it applies to HX-MS protocols, the practice of HX-MS including data analysis and interpretation is explained in detail, and recent advancements in technology which greatly increase the depth of information gained from the technique are highlighted.

References

Apr 1, 1991·Rapid Communications in Mass Spectrometry : RCM·V Katta, B T Chait
May 15, 1985·Analytical Biochemistry·J J EnglanderS W Englander
Jan 18, 1972·Biochemistry·R S MoldayR G Kallen
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Oct 29, 1982·Molecular and Cellular Biochemistry·C WoodwardE Tüchsen
Sep 1, 1994·Proteins·Y BaiS W Englander
Sep 1, 1993·Proteins·Y BaiS W Englander
Apr 1, 1993·Protein Science : a Publication of the Protein Society·Z Zhang, D L Smith
Feb 1, 1997·Journal of Mass Spectrometry : JMS·D L SmithZ Zhang
Oct 24, 1998·Analytical Chemistry·J G MandellE A Komives
Aug 10, 1999·Journal of the American Society for Mass Spectrometry·Y DengD L Smith
Aug 19, 1999·Protein Science : a Publication of the Protein Society·R Li, C Woodward
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Lintao WangDavid L Smith
Sep 13, 2002·Journal of the American Chemical Society·Jeroen A A DemmersAlbert J R Heck
Feb 25, 2003·Annual Review of Biophysics and Biomolecular Structure·Andrew N HoofnagleNatalie G Ahn
May 28, 2003·Mass Spectrometry Reviews·Lars Konermann, Douglas A Simmons
Aug 1, 1954·Biochimica Et Biophysica Acta·A HVIDT, K LINDERSTRØM-LANG
Apr 20, 2004·Assay and Drug Development Technologies·Ricardo A GarciaFrancisco J Villarreal
Jun 17, 2004·Journal of the American Chemical Society·Joshua K HoernerIgor A Kaltashov
Feb 24, 2005·Journal of the American Chemical Society·Thomas J D JørgensenPeter Roepstorff
May 11, 2005·Protein Science : a Publication of the Protein Society·Lintao Wang, David L Smith
Oct 7, 2005·Mass Spectrometry Reviews·Thomas E Wales, John R Engen
Jan 13, 2006·Journal of the American Society for Mass Spectrometry·Yan WuWilliam B Hobbins
Feb 21, 2006·Journal of Molecular Biology·Thomas E Wales, John R Engen
Aug 1, 2006·Journal of the American Society for Mass Spectrometry·David D WeisLynn F Ten Eyck
Aug 26, 2006·Journal of the American Society for Mass Spectrometry·David D WeisIgnatius J Kass
Mar 11, 2008·Rapid Communications in Mass Spectrometry : RCM·Yoshitomo HamuroJeffrey A Morrow
Aug 5, 2008·Analytical Chemistry·Thomas E WalesJohn R Engen

❮ Previous
Next ❯

Citations

Mar 3, 2010·Analytical and Bioanalytical Chemistry·Sean R Marcsisin, John R Engen
Aug 2, 2013·Journal of Virology·Thaddeus M DavenportKelly K Lee
May 13, 2014·The Biochemical Journal·Dionysios PantazatosGary E Gilbert
Oct 15, 2014·Expert Opinion on Drug Discovery·Gengyang YuanGraham B Jones
Apr 10, 2014·Protein Science : a Publication of the Protein Society·Jessica Z BereszczakAlbert J R Heck
Sep 3, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kevin B Dagbay, Jeanne A Hardy
Apr 7, 2018·The Journal of Biological Chemistry·Aaron A CookWolfgang Bergmeier
Apr 13, 2019·Nature Communications·Ingvar R MöllerKasper D Rand
Aug 19, 2020·Nature Chemical Biology·Ann M CathcartLoren D Walensky
Sep 8, 2016·Mass Spectrometry Reviews·Jürgen Claesen, Tomasz Burzykowski
Feb 6, 2017·The Journal of Biological Chemistry·Kevin B DagbayJeanne A Hardy
Apr 20, 2013·Journal of the American Chemical Society·Jessica Z BereszczakAlbert J R Heck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.