Investigating the assembly of the bacterial type III secretion system injectisome by in vivo photocrosslinking

International Journal of Medical Microbiology : IJMM
Nidhi Singh, Samuel Wagner

Abstract

Virulence-associated type III secretion systems serve the injection of bacterial effector proteins into eukaryotic host cells. These effector proteins modulate host cell biology in order to promote colonization and infection, hence type III secretion systems are often essential bacterial pathogenicity factors. The core of type III secretion systems is a cell envelope-spanning macromolecular machine called injectisome. It consists of almost twenty different components in a stoichiometry of one to more than one hundred. Assembly of this 6 MDa complex requires the coordinated integration of components from the cytoplasm, the inner membrane, the periplasm, the outer membrane and even the extracellular space of Gram-negative bacteria. Here, we review injectisome assembly with an emphasis on the techniques that were employed towards its investigation. In particular, we focus on in vivo photocrosslinking, a technique that exploits the encoding of the artificial UV-inducible crosslinking amino acid p-benzoyl-phenylalanine to identify protein-protein interactions and to delineate assembly pathways.

References

Jul 20, 1992·Journal of Molecular Biology·T KuboriS Aizawa
Feb 15, 1983·Journal of Molecular Biology·J H Miller, A M Albertini
Aug 1, 1994·Journal of Bacteriology·A AllaouiG R Cornelis
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·T G Kimbrough, S I Miller
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason W ChinPeter G Schultz
Sep 21, 2005·Nature Methods·Ian S FarrellJason W Chin
Oct 7, 2005·Nature·Yukihiro Akeda, Jorge E Galán
Oct 26, 2005·The Journal of Biological Chemistry·Hedda U FerrisRobert M Macnab
Apr 5, 2007·Nature Protocols·Ilka WittigHermann Schägger
Aug 19, 2007·Molecular Microbiology·Petr BrozGuy R Cornelis
Jul 29, 2008·FEMS Microbiology Letters·Steven Johnson, Ariel Blocker
Nov 4, 2008·Journal of Molecular Biology·Ulrich WiesandDirk W Heinz
Jan 24, 2009·Protein Science : a Publication of the Protein Society·George T LountosDavid S Waugh
May 11, 2010·The EMBO Journal·Andreas DiepoldGuy R Cornelis
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gert BangeIrmgard Sinning
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samuel WagnerJorge E Galán
Jan 20, 2011·Molecular Microbiology·Hui Li, Victor Sourjik
Feb 1, 2011·Nature Structural & Molecular Biology·Tatsuya IbukiKeiichi Namba
Mar 10, 2011·Science·Oliver Schraidt, Thomas C Marlovits
Sep 1, 2011·The Journal of Biological Chemistry·Xiu-Jun YuDavid W Holden
Sep 22, 2011·Nature Communications·Tohru MinaminoKeiichi Namba
Jun 16, 2012·Nature·Antoine LoquetAdam Lange
Jul 14, 2012·Molecular Microbiology·Andreas DiepoldGuy R Cornelis
Dec 12, 2012·Nature Structural & Molecular Biology·Patrizia AbrusciSusan M Lea
Mar 5, 2013·Protein Science : a Publication of the Protein Society·Nicholas E DickensonWilliam D Picking
Nov 12, 2013·Nature·Lewis D B EvansGillian M Fraser
Jan 24, 2014·Molecular Microbiology·Yusuke V MorimotoTohru Minamino
Feb 4, 2014·FEMS Microbiology Reviews·Andreas Diepold, Samuel Wagner
Jul 9, 2014·Annual Review of Microbiology·Jorge E GalánSamuel Wagner
Nov 14, 2014·PLoS Genetics·Marc ErhardtKelly T Hughes
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bo HuJun Liu

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