Investigating the authenticity of Ophiopogonis Radix and its Chinese patent medicines by using a nucleotide signature.

Journal of Ethnopharmacology
Mengyue GuoXiaohui Pang

Abstract

Ophiopogonis Radix (Maidong), derived from the dried root tuber of Ophiopogon japonicus (Thunb.) Ker Gawl., has been widely used in the treatment of chronic inflammatory and cardiovascular diseases. However, Ophiopogonis Radix is often adulterated with some species because of morphological similarities. Adulterants circulating in herbal markets are a latent threat to the clinical safety and consumers' interest. We aimed to develop a nucleotide signature for identification of Ophiopogonis Radix and its Chinese patent medicines. A total of 255 ITS2 sequences representing 39 species and 4 varieties were used to develop a nucleotide signature of Ophiopogonis Radix. The nucleotide signature was used to investigate 17 commercial crude drugs and eight batches of Chinese patent medicines. A 69 bp nucleotide signature unique to Ophiopogonis Radix was found. The survey revealed that 2 of 17 crude drug samples were adulterants detected as Liriopes Radix (Shanmaidong). Fortunately, no adulterants were detected in the eight batches of Chinese patent medicines. The newly developed nucleotide signature could be efficiently applied to identify Ophiopogonis Radix and its Chinese patent medicines, aiding in the authentication, quality control, a...Continue Reading

References

Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
May 14, 2008·BMC Genomics·Isabelle MeusnierMehrdad Hajibabaei
Nov 26, 2008·Gene·Alexander KellerMatthias Wolf
Jun 2, 2012·Genetics and Molecular Research : GMR·G Li, Y-J Park
Dec 13, 2012·Scientific Reports·Rong ChenZhuo Tang
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 14, 2014·Molecular Biotechnology·Belinda Ling Nah NgTiong Chia Yeo
Aug 5, 2014·Biotechnology Advances·Shilin ChenChristine Leon
Feb 7, 2015·Chinese Medicine·Yat-Tung LoPang-Chui Shaw
Jul 2, 2015·Journal of Natural Medicines·Michiho ItoKatsuyuki Matsumura
Oct 27, 2015·Frontiers in Plant Science·Sha ZhaoShilin Chen
Jan 31, 2016·Journal of Ethnopharmacology·Min-Hui ChenYi-Tao Wang
Jul 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Li-Li JiangPang-Chui Shaw
Nov 29, 2017·Frontiers in Plant Science·Mengyue GuoXiaohui Pang
Apr 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingjing ZhangShilin Chen
Nov 23, 2019·Scientific Reports·Hongying DuanYanqing Zhou
Nov 25, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·YuShan TianJin Qi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.