Investigating the chemical profile of regenerated scorpion (Parabuthus transvaalicus) venom in relation to metabolic cost and toxicity

Toxicon : Official Journal of the International Society on Toxinology
Zia NisaniWilliam K Hayes

Abstract

We investigated the biochemical profile of regenerated venom of the scorpion Parabuthus transvaalicus in relation to its metabolic cost and toxicity. Using a closed-system respirometer, we compared oxygen consumption between milked and unmilked scorpions to determine the metabolic costs associated with the first 192 h of subsequent venom synthesis. Milked scorpions had a substantially (21%) higher mean metabolic rate than unmilked scorpions, with the largest increases in oxygen consumption occurring at approximately 120 h, 162 h, and 186 h post-milking. Lethality tests in crickets indicated that toxicity of the regenerated venom returned to normal levels within 4 d after milking. However, the chemical profile of the regenerated venom, as evaluated by FPLC and MALDI-TOF mass spectrometry, suggested that regeneration of different venom components was asynchronous. Some peptides regenerated quickly, particularly those associated with the scorpion's "prevenom," whereas others required much or all of this time period for regeneration. This asynchrony could explain the different spikes detected in oxygen consumption of milked scorpions as various peptides and other venom components were resynthesized. These observations confirm the r...Continue Reading

References

Jan 1, 1979·Toxicon : Official Journal of the International Society on Toxinology·A Yahel-Niv, E Zlotkin
Apr 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·M J PaineJ M Crampton
Dec 1, 1969·Toxicon : Official Journal of the International Society on Toxinology·E Zlotkin, A S Shulov
Oct 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·J L BoevéW Nentwig
Jan 15, 1998·Toxicon : Official Journal of the International Society on Toxinology·E Kalapothakis, C Chávez-Olórtegui
Jun 10, 1998·Toxicon : Official Journal of the International Society on Toxinology·T DebontJ Tytgat
Sep 22, 1999·European Journal of Biochemistry·L D PossaniJ Tytgat
Aug 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·M AlamiM F Martin-Eauclaire
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·Esther WiggerWolfgang Nentwig
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bora InceogluBruce D Hammock
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Bora InceogluBruce D Hammock
Nov 9, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ricardo C Rodríguez de la Vega, Lourival D Possani
Mar 9, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Zia NisaniWilliam K Hayes
Oct 24, 2007·Toxicon : Official Journal of the International Society on Toxinology·L H du PlessisJ L du Plessis
Dec 24, 2008·Toxicon : Official Journal of the International Society on Toxinology·Mohamed A Abdel-RahmanAlistair McVean
Jul 28, 2010·Toxicon : Official Journal of the International Society on Toxinology·Anna F V PintorJamie E Seymour

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Citations

Dec 3, 2014·Zoology : Analysis of Complex Systems, ZACS·Allen M CooperWilliam K Hayes
Dec 20, 2013·Toxins·Timothy N W JacksonBryan G Fry
Feb 20, 2014·Toxicon : Official Journal of the International Society on Toxinology·Allen M CooperWilliam K Hayes
Dec 26, 2012·Toxicon : Official Journal of the International Society on Toxinology·David Morgenstern, Glenn F King
Apr 29, 2015·Toxicon : Official Journal of the International Society on Toxinology·Arie van der MeijdenMykola Rasko
Mar 10, 2015·Behavioural Processes·Zia Nisani, William K Hayes
Feb 10, 2018·ELife·Yaara Y Columbus-ShenkarYehu Moran
May 16, 2019·Proceedings. Biological Sciences·Ryann A BlennerhassettGregory P Brown
Mar 13, 2018·Frontiers in Pharmacology·Jessica M de SouzaFabiola M Ribeiro
Oct 5, 2017·PloS One·Thomas McElroyEric A Albrecht
Apr 13, 2021·Ecology and Evolution·Linda Hernández DuranTasmin Lee Rymer

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