Investigating the effect of design parameters on the response time of a highly sensitive microbial hydrogen sulfide biosensor based on oxygen consumption

Biosensors & Bioelectronics
Amin VosoughiMaziar Hakim

Abstract

A novel hydrogen sulfide microbial biosensor was developed based on investigating the influence of four design parameters: cell concentration, immobilization bed type, hydrogen sulfide concentration, and geometrical shape of the biosensor. Thiobacillus thioparus was used as the recognition element and it was immobilized on sodium alginate as well as agarose bed. The results were optimized by the application of statistical optimization software based on response time of the system. Oxygen reduction was considered as the detection sign. Sodium alginate solution with a concentration of 2.3% (w/v) and optical density of 10 at 605nm was found as the optimum conditions for immobilization with response time of 72s. Optimum response time of immobilized T. thioparus on agarose was also found equal to 120s at agarose concentration of 1.2% (w/v) and optical density of 10.83. Performance of the biosensor in different temperatures, pH and agitation speeds was also analyzed. The designed biosensor could detect concentrations of hydrogen sulfide as low as 0.5ppm. T. thioparus could retain 99% of the original activity in both systems, after ten days passing the fabrication. A fractal analysis was also done theoretically to investigate the diff...Continue Reading

References

Jan 7, 2000·Annals of Biomedical Engineering·J J PancrazioD A Stenger
Apr 28, 2004·Biophysical Journal·Nicolas Fatin-RougeJacques Buffle

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Citations

Jan 23, 2019·Biotechnology and Applied Biochemistry·Amirhossein GhaemiOmid Asayeshnaeini
Jun 27, 2019·Biosensors & Bioelectronics·Niharika GuptaBansi D Malhotra

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