PMID: 12763773May 24, 2003Paper

Investigating the energy transduction mechanism of P-type ATPases with Fe2+-catalyzed oxidative cleavage

Annals of the New York Academy of Sciences
Steven J D Karlish

Abstract

This paper discusses specific oxidative cleavage of renal Na(+),K(+)-ATPase and gastric H(+),K(+)-ATPase, catalyzed by bound Fe(2+) or the complex ATP-Fe(2+), and its implication for the energy transduction mechanism of P-type ATPases. The cleavage technique provides information on the spatial organization of the proteins in different conformations and, since ATP-Fe(2+) substitutes for ATP-Mg(2+) in activating ATPase activity, on Mg(2+)-ligating residues in different conformations. The experiments predict the existence of large movements of N, P, and A cytoplasmic domains accompanying E(1)-E(2) and E(1) P-E(2)P conformational transitions-open in E(1) conformations and closed in E(2) conformations. These features fit well with the Ca(2+)-ATPase crystal structures in E(1) or E(2) conformations and also provide evidence on ATP and Mg(2+) binding sites that is not available from the structures. By combining information from cleavage experiments with molecular modeling, based on the Ca(2+)-ATPase structure, features such as an N to P domain interaction in an E(1). ATP-Mg(2+) conformation can be inferred. The organization of the N, P, and A domains and the ATP and Mg(2+) binding sites in the different conformational states appears to...Continue Reading

References

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May 24, 2003·Annals of the New York Academy of Sciences·David StrugatskySteven J D Karlish

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Citations

Sep 4, 2009·The Journal of Biological Chemistry·Talya BelogusSteven J D Karlish
Jan 21, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Malson N LucenaFrancisco A Leone
Jan 8, 2018·Annals of Hematology·Leilismara SousaLeandro Augusto Barbosa
Jul 21, 2004·The Journal of Biological Chemistry·Cedric MontignyMarc le Maire
Dec 4, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Leilismara SousaLeandro A Barbosa
Apr 6, 2005·Biochemistry·Keith MunsonGeorge Sachs
Sep 30, 2018·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Leonardo M FabriFrancisco A Leone

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