Investigating the genetic basis of pork tenderness: genomic analysis of porcine CAST

Animal Genetics
Stacey N Meyers, Jonathan E Beever

Abstract

A quantitative trait locus (QTL) affecting pork tenderness was recently detected within the Illinois Meat Quality Pedigree (IMQP) and fine-mapped to the region of porcine chromosome 2 (SSC2) harbouring the functional candidate gene calpastatin (CAST). To identify molecular variation that may underlie the observed differences in tenderness phenotypes, we characterized the porcine CAST gene and analysed allelic variation within the F(1) boars of the IMQP. The complete genomic sequence of porcine CAST has been determined, and was found to contain 35 exons spanning nearly 123 kb. Using the rapid amplification of cDNA ends (RACE) method, calpastatin transcript types I-III, as well as a putative novel transcript type, were detected within porcine skeletal muscle. Variability in transcription initiation and termination sites was observed, and alternative splicing of exons 1y and 3 was evident. Nearly 77.6% of the CAST gene, including all exons, was re-sequenced from each of six IMQP F(1) boars, and almost 900 polymorphisms were identified. The heterozygosity of nearly 400 polymorphisms appeared to be concordant with the previous QTL data, and the location of this variation within the CAST gene suggests that a causative mutation is lik...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y EmoriK Suzuki
Jun 23, 1995·Journal of Molecular Biology·D S Prestridge
Mar 1, 1994·Journal of Animal Science·J Killefer, M Koohmaraie
Feb 25, 1998·FEBS Letters·R De TullioS Pontremoli
Jan 23, 1999·Nature Genetics·A Chakravarti
Mar 13, 1999·The Journal of Biological Chemistry·H JiangM C Lucy
Jul 15, 1999·Biochemical and Biophysical Research Communications·J TakanoM Maki
Apr 26, 2000·Genome Research·S SchwartzW Miller
Jun 3, 2000·Nature Genetics·B Ewing, P Green
Oct 25, 2001·Archives of Biochemistry and Biophysics·T ParrP J Buttery
May 29, 2002·Animal Genetics·S C FahrenkrugJ W Keele
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark S SpringerStephen J O'Brien
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Mar 23, 2004·Journal of Animal Science·M P KentM Koohmaraie
Jun 5, 2004·Archives of Biochemistry and Biophysics·Tim ParrPeter J Buttery
Jul 17, 2004·Biological Chemistry·Amanda WendtDarrel E Goll
Oct 16, 2004·Journal of Animal Science·D C CiobanuM F Rothschild
Apr 12, 2005·Archives of Biochemistry and Biophysics·Peggy RaynaudValérie Amarger
Jul 13, 2005·Archives of Biochemistry and Biophysics·Peggy RaynaudHubert Levéziel
Jan 31, 2006·Animal Genetics·G A RohrerM Koohmaraie
Sep 15, 2006·Journal of Animal Science·G H GeesinkM Koohmaraie
Jun 6, 2007·Yi chuan = Hereditas·Xiu-Qin YangDi Liu
Jun 16, 2007·Archives of Biochemistry and Biophysics·Roberta De TullioEdon Melloni
Oct 16, 2007·BMC Genetics·Stacey N MeyersJonathan E Beever

❮ Previous
Next ❯

Citations

May 29, 2010·BMC Genomics·Stacey N MeyersTimothy P L Smith
Mar 4, 2014·Meat Science·K Ropka-MolikT Szmatoła
Sep 18, 2008·Current Opinion in Otolaryngology & Head and Neck Surgery·Maria Sol ColladoJeffrey T Corwin
Apr 9, 2010·Meat Science·Caroline M KempTim Parr
Mar 1, 2013·Asian-Australasian Journal of Animal Sciences·Ting LianYiping Liu
Dec 8, 2015·Journal of Animal Science·Y L Bernal RubioJ P Steibel
Oct 30, 2015·Bioinformatics and Biology Insights·Dominic Wright
Nov 21, 2018·PloS One·María MuñozCristina Óvilo
Apr 2, 2011·Journal of Animal Science·D NonnemanS J Moeller
Aug 15, 2013·Journal of Animal Science·D J NonnemanG A Rohrer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.