Investigating the interaction mechanism between zinc and Saccharomyces cerevisiae using combined SEM-EDX and XAFS

Applied Microbiology and Biotechnology
Can Chen, Jianlong Wang

Abstract

The interaction mechanism between zinc and the intact yeast cells of Saccharomyces cerevisiae was investigated by using the scanning electron microscopy with energy-dispersive X-ray analysis, as well as X-ray absorption fine structure spectroscopy (XAFS). Displacement of H+, K+, Mg2+, and Na+ during zinc uptake confirmed the existence of both covalent interactions and ionic interactions between Zn2+ and the microbe. Ion exchange mechanism played a role in zinc uptake. The local environment of Zn accumulated in the intact yeast cells was determined by XAFS, which suggests that the nearest neighboring atom of the bound zinc ion on the biomass is oxygen atom. The adsorbed zinc ion on the intact cells of S. cerevisiae is a tetrahedron structure, with the Zn-O bond length of 1.97 A, and the coordination number is only 3.2 of Zn-O structure in the first shell.

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Citations

Jul 5, 2013·Applied Microbiology and Biotechnology·Eduardo V Soares, Helena M V M Soares
Dec 24, 2008·Biotechnology Advances·Jianlong Wang, Can Chen
Jan 9, 2014·Journal of Environmental Health Science & Engineering·Geetanjali BasakNilanjana Das
May 29, 2016·Journal of Environmental Radioactivity·Can Chen, Jianlong Wang
Sep 9, 2017·Critical Reviews in Food Science and Nutrition·Derong LinSuqing Li
Oct 8, 2020·Heliyon·Manal T El Sayed, Ashraf S A El-Sayed
Nov 17, 2019·Food Research International·Valéria Rampazzo RibeiroCharles Windson Isidoro Haminiuk

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