Investigating the Radioresistant Properties of Lung Cancer Stem Cells in the Context of the Tumor Microenvironment

Radiation Research
Ryan ChanMeenakshi Upreti

Abstract

Lung cancer is the most common cause of cancer-related deaths worldwide and non-small cell lung cancer (NSCLC) accounts for ~85% of all lung cancer. While recent research has shown that cancer stem cells (CSC) exhibit radioresistant and chemoresistant properties, current cancer therapy targets the bulk of the tumor burden without accounting for the CSC and the contribution of the tumor microenvironment. CSC interaction with the stroma enhances NSCLC survival, thus limiting the efficacy of treatment. The aim of this study was to elucidate the role of CSC and the microenvironment in conferring radio- or chemoresistance in an in vitro tumor model for NSCLC. The novel in vitro three-dimensional (3D) NSCLC model of color-coded tumor tissue analogs (TTA) that we have developed is comprised of human lung adenocarcinoma cells, fibroblasts, endothelial cells and NSCLC cancer stem cells maintained in low oxygen conditions (5% O2) to recapitulate the physiologic conditions in tumors. Using this model, we demonstrate that a single 5 Gy radiation dose does not inhibit growth of TTA containing CSC and results in elevated expression of cytokines (TGF-α, RANTES, ENA-78) and factors (vimentin, MMP and TIMP), indicative of an invasive and aggres...Continue Reading

References

Nov 1, 1973·Journal of the National Cancer Institute·D J GiardW P Parks
Aug 6, 1998·The Journal of Clinical Investigation·D A ArenbergR M Strieter
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·R K Jain, N S Forbes
Apr 12, 2002·Oncogene·Yangfu JiangY Eric Shi
Jul 12, 2002·Current Gene Therapy·Karsten Brand
Jul 4, 2003·The Journal of Clinical Investigation·Guénahel H DanetM Celeste Simon
Jul 14, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ibrahim S AljadaDongfeng Tan
Jan 15, 2005·Annals of the New York Academy of Sciences·Robert M StrieterMichael P Keane
Dec 21, 2006·Journal of the National Cancer Institute·Maximilian Diehn, Michael F Clarke
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
Oct 6, 2007·Chest·Lary A RobinsonUNKNOWN American College of Chest Physicians
Nov 21, 2007·Current Opinion in Biotechnology·Laurie E Ailles, Irving L Weissman
May 31, 2008·Nature Reviews. Cancer·Michael BaumannRichard Hill
Jan 23, 2009·Journal of Cellular Physiology·Zoran Ivanovic
Sep 3, 2009·Cell Structure and Function·Kaori TsutsumiYusuke Ohba
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Giulia BertoliniGabriella Sozzi
Mar 31, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anne AupérinJean-Pierre Pignon
Jun 10, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Catherine Adell O'BrienCatriona H M Jamieson
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Mar 15, 2012·Cancer Research·Melody A SwartzYves A DeClerck
Aug 29, 2012·Nature Reviews. Clinical Oncology·Allan Price
Oct 24, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Meenakshi UpretiRobert J Griffin
Apr 9, 2013·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Maryline DauphinMyriam Polette
Jan 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jun YeJian Huang
Jan 21, 2014·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Amar DesaiStanton L Gerson
Jan 21, 2014·Biochimica Et Biophysica Acta·Brett BurkholderRuo-Pan Huang
Feb 14, 2014·Mediators of Inflammation·Donatella Aldinucci, Alfonso Colombatti
May 1, 2009·International Journal of Stem Cells·Yonghyun KimDo-Hyun Nam
Nov 22, 2014·Current Opinion in Oncology·Eva FaurobertCorinne Albiges-Rizo
Dec 17, 2014·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Mingchuan ZhaoCaicun Zhou
Mar 11, 2015·Journal of Neuropathology and Experimental Neurology·Mumtaz V RojianiAmyn M Rojiani

❮ Previous
Next ❯

Citations

Jun 17, 2016·Cancer Biotherapy & Radiopharmaceuticals·Guo-Ping ShanYi-Wei Yang
Jun 24, 2017·Carcinogenesis·Neha MerchantArif N Ali
Jul 13, 2019·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·T B SteinbichlerJ Dudas
Feb 10, 2018·Oncotarget·Teresa Bernadette SteinbichlerSkvortsova Ira-Ida
Sep 17, 2020·Biotechnology Letters·Qiao Chen, Youbin Wang
Jul 30, 2017·International Journal of Molecular Sciences·Dimakatso Alice SenthebaneKevin Dzobo
Feb 14, 2018·Molecular Medicine Reports·Jiangzhou PengYihui Lin
Apr 25, 2018·Journal of Experimental & Clinical Cancer Research : CR·Le TangZhaoyang Zeng
Sep 27, 2019·Frontiers in Oncology·Jie NiYong Li
Jul 1, 2017·Targeted Oncology·Justin D Lathia, Huiping Liu
Jun 27, 2019·Cancers·Alexander SchulzKerstin Borgmann
Nov 6, 2018·International Journal of Oncology·Shuang WangQiang Liu
May 18, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Narumol BhummaphanPithi Chanvorachote

❮ Previous
Next ❯

Related Concepts

© 2021 Meta ULC. All rights reserved