Investigating the requirement for calcium during lactococcal phage infection

International Journal of Food Microbiology
Jennifer MahonyDouwe van Sinderen

Abstract

Calcium is widely used in the study and successful propagation of virulent bacteriophages infecting lactic acid bacteria; however, it has not been assessed if and why this divalent cation is required for the infection process. Lactococcal phages are a persistent problem in the calcium-rich dairy environment and therefore were used as the model for this study. Using representative members of nine of the currently recognized ten lactococcal phage groups, encompassing phages of the Podoviridae and Siphoviridae families, we present data to suggest that calcium is not an explicit requirement for many of these phages. However, calcium expedited the pace of the lytic cycle for certain phages. Additionally, for calcium-dependent phages belonging to the 936 siphogroup, we could substitute this cation with magnesium or manganese, indicating that these phages are more adaptable than lactococcal phages of other groups. We postulate that the ability of phages to adapt to their environment and to harness the available mineral content may ultimately decide the success of a given phage infection. This may explain, in part, why 936 phages are one of the most frequently isolated phages in the dairy industry.

References

Feb 1, 1979·The Journal of General Virology·H Y Steensma, J Blok
Oct 1, 1979·Canadian Journal of Microbiology·M L SaxelinV Sundman
Oct 1, 1972·The Journal of General Virology·K Watanabe, S Takesue
Mar 29, 1974·Biochimica Et Biophysica Acta·W SnipesA Davis
Jun 6, 2006·Applied and Environmental Microbiology·Hélène DeveauSylvain Moineau
Oct 7, 2006·Biochimica Et Biophysica Acta·Robert J P Williams
May 1, 2007·Virology·Alain ChopinMarie-Christine Chopin
Feb 7, 2009·International Journal of Food Microbiology·Andrei Raiski, Natalya Belyasova
Jan 12, 2010·Applied and Environmental Microbiology·Marie-Eve Dupuis, Sylvain Moineau
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Giuliano SciaraChristian Cambillau
Aug 31, 2010·Applied and Environmental Microbiology·Julie E Samson, Sylvain Moineau
Dec 22, 2011·Journal of Food Protection·Therese DeasyDouwe van Sinderen
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·David VeeslerChristian Cambillau
Aug 28, 2013·Nature Reviews. Microbiology·Julie E SamsonSylvain Moineau
May 30, 2014·Journal of Virology·Silvia SpinelliChristian Cambillau
Sep 5, 2014·Microbial Cell Factories·Jennifer MahonyGerald F Fitzgerald
Sep 7, 2014·Applied and Environmental Microbiology·Simon J LabrieSylvain Moineau

❮ Previous
Next ❯

Citations

Jan 7, 2016·Annual Review of Food Science and Technology·Jennifer MahonyDouwe van Sinderen
Oct 20, 2015·International Journal of Food Microbiology·Lynn El HaddadSylvain Moineau
Nov 9, 2017·Antibiotics·Lucía FernándezAna Rodríguez
Aug 7, 2018·Journal of Applied Microbiology·S A PujatoD J Mercanti
Jun 9, 2020·Environmental Microbiology·Alessandra G de MeloSylvain Moineau
Feb 12, 2017·BMC Genomics·Jennifer MahonyDouwe van Sinderen
Mar 11, 2019·Journal of Dairy Science·X ChenB Munguntsetseg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.