Investigating the Role of G Protein βγ in Kv7-Dependent Relaxations of the Rat Vasculature.

Arteriosclerosis, Thrombosis, and Vascular Biology
Jennifer B StottIain A Greenwood

Abstract

Objective- In renal arteries, inhibitors of G protein βγ subunits (Gβγ) reduce Kv7 activity and inhibit Kv7-dependent receptor-mediated vasorelaxations. However, the mechanisms underlying receptor-mediated relaxation are artery specific. Consequently, the aim of this study was to ascertain the role of Gβγ in Kv7-dependent vasorelaxations of the rat vasculature. Approach and Results- Isometric tension recording was performed in isolated rat renal, mesenteric, and cerebral arteries to study isoproterenol and calcitonin gene-related peptide relaxations. Kv7.4 was knocked down via morpholino transfection while inhibition of Gβγ was investigated with gallein and M119K. Proximity ligation assay was performed on isolated myocytes to study the association between Kv7.4 and G protein β subunits or signaling intermediaries. Isoproterenol or calcitonin gene-related peptide-induced relaxations were attenuated by Kv7.4 knockdown in all arteries studied. Inhibition of Gβγ with gallein or M119K had no effect on isoproterenol-mediated relaxations in mesenteric artery but had a marked effect on calcitonin gene-related peptide-induced responses in mesenteric artery and cerebral artery and isoproterenol responses in renal artery. Isoproterenol in...Continue Reading

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Citations

Apr 25, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Jun ChengYan Yang
Dec 24, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Hong S LuAlan Daugherty
Jul 7, 2020·The Journal of Physiology·Miguel A S Martín-Aragón BaudelAnthony P Albert
Aug 14, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Vincenzo BarreseIain A Greenwood
Jul 23, 2020·Frontiers in Physiology·Jennifer van der HorstThomas A Jepps
Jan 30, 2020·Frontiers in Physiology·Iain A Greenwood, Jennifer B Stott
Oct 13, 2020·Frontiers in Physiology·John Malysz, Georgi V Petkov
Feb 4, 2021·The Journal of General Physiology·Jennifer van der HorstThomas A Jepps
Feb 19, 2020·Pharmacology & Therapeutics·Boris ManouryRodolphe Fischmeister
Jan 11, 2022·American Journal of Physiology. Renal Physiology·Dieniffer Peixoto-NevesAdebowale Adebiyi

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