Investigating the role of H₂S in 4-HNE scavenging

Methods in Enzymology
Hilde Laggner, Bernhard M K Gmeiner

Abstract

4-HNE (4-hydroxy-2-nonenal) is a highly reactive α,β-unsaturated aldehyde generated from oxidation of polyunsaturated fatty acids and has been suggested to play a role in the pathogenesis of several diseases. 4-HNE can bind to amino acids, proteins, polynucleotides, and lipids and exert cytotoxicity. 4-HNE forms adducts (Michael adducts) with cysteine, lysine, as well as histidine on proteins with the thiol function as the most reactive nucleophilic moiety. Thus, detoxification strategies by 4-HNE scavenging compounds might be of interest. Recently, hydrogen sulfide (H2S) has been identified as an endogenous vascular gasotransmitter and neuromodulator. Assuming that the low-molecular thiol H2S may react with 4-HNE, methods to monitor the ability of H2S to counteract the protein-modifying and cytotoxic activity of 4-HNE are described in this chapter.

Citations

Aug 24, 2017·Reproduction in Domestic Animals = Zuchthygiene·P Martín MuñozC Ortega Ferrusola
Aug 26, 2021·Antioxidants & Redox Signaling·Clément CrochemoreMiria Ricchetti

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