Investigating the specific uptake of EGF-conjugated nanoparticles in lung cancer cells using fluorescence imaging

Cancer Nanotechnology
Honglin JinGang Zheng

Abstract

Targeted nanoparticles have the potential to deliver a large drug payload specifically to cancer cells. Targeting requires that a ligand on the nanoparticle surface interact with a specific membrane receptor on target cells. However, the contribution of the targeting ligand to nanoparticle delivery is often influenced by non-specific nanoparticle uptake or secondary targeting mechanisms. In this study, we investigate the epidermal growth factor (EGF) receptor-targeting specificity of a nanoparticle by dual-color fluorescent labeling. The targeted nanoparticle was a fluorescently labeled, EGF-conjugated HDL-like peptide-phospholipid scaffold (HPPS) and the cell lines expressed EGF receptor linked with green fluorescent protein (EGFR-GFP). Using LDLA7 cells partially expressing EGFR-GFP, fluorescence imaging demonstrated the co-internalization of EGFR-GFP and EGF-HPPS, thus validating its targeting specificity. Furthermore, specific EGFR-mediated uptake of the EGF-HPPS nanoparticle was confirmed using human non-small cell lung cancer A549 cells. Subsequent confocal microscopy and flow cytometry studies delineated how secondary targeting mechanisms affected the EGFR targeting. Together, this study confirms the EGFR targeting of EG...Continue Reading

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Citations

Oct 13, 2017·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Ali DabbaghNoorsaadah Abdul Rahman
Dec 20, 2012·Nanomedicine·Alyssa M Master, Anirban Sen Gupta
Oct 1, 2012·Journal of Innovative Optical Health Sciences·Trevor D McKeeRama Khokha
Feb 27, 2018·Chemical Society Reviews·Christopher D SpicerMolly M Stevens
Sep 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dennis Pedersbæk, Jens B Simonsen
May 12, 2011·Accounts of Chemical Research·Kenneth K NgGang Zheng

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Methods Mentioned

BETA
gel filtration
transfections
flow cytometry
confocal microscopy
transfection
fluorescence imaging
confocal

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