Investigating the underlying mechanism of Saccharomyces cerevisiae in response to ethanol stress employing RNA-seq analysis

World Journal of Microbiology & Biotechnology
Ruoyun LiPeifang Weng

Abstract

Saccharomyces cerevisiae has been widely used for wine fermentation and bio-fuels production. A S. cerevisiae strain Sc131 isolated from tropical fruit shows good fermentation properties and ethanol tolerance, exhibiting significant potential in Chinese bayberry wine fermentation. In this study, RNA-sequence and RT-qPCR was used to investigate the transcriptome profile of Sc131 in response to ethanol stress. Scanning Electron Microscopy were carried out to observe surface morphology of yeast cells. Totally, 937 genes were identified differential expressed, including 587 up-regulated and 350 down-regulated genes, after 4-h ethanol stress (10% v/v). Transcriptomic analysis revealed that, most genes involved in regulating filamentous growth or pseudohyphal growth were significantly up-regulated in response to ethanol stress. The complex protein quality control machineries, Hsp90/Hsp70 and Hsp104/Hsp70/Hsp40 based chaperone system combining with ubiquitin-proteasome proteolytic pathway were both activated to recognize and degrade misfolding proteins. Genes related to biosynthesis and metabolism of two well-known stress-responsive substances trehalose and ergosterol were generally up-regulated, while genes associated with amino acid...Continue Reading

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Citations

Oct 20, 2018·Frontiers in Bioengineering and Biotechnology·Leonardo Martins-SantanaRafael Silva-Rocha
Mar 7, 2020·Food Microbiology·Juan A Porras-AgüeraTeresa García-Martínez
Nov 24, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Yueqi LiZufang Wu

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Methods Mentioned

BETA
RNA-seq
scanning electron microscopy
Assay
RNA Assay
electrophoresis
PCR
2500 sequencing
protein folding
environmental stresses
ubiquitination

Software Mentioned

Blast2
STRING
Pathway Tools

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