Investigating transport proteins by solid state NMR

Naunyn-Schmiedeberg's Archives of Pharmacology
Daniel BastingClemens Glaubitz

Abstract

Transporters form an interesting and complex class of membrane proteins. Many of them are potential drug targets due to their role in translocation of ions, small molecules and peptides across the membrane or due to their role in multidrug resistance. Hence elucidating their structure and mechanism is of great importance and may lead to a host of new drugs and methods to alter or inhibit their function. Solid state NMR is an emerging technique for investigating transport proteins. Along with other biochemical and biophysical techniques solid state NMR can provide data on drug binding, protein dynamics and structure at the interface between structural biology and functional analysis. Here, we review solid state NMR applications to primary active and secondary transporters involved in translocation of small molecules. We discuss current experimental limitations and give an overall perspective on how the technique may be used to address some pertinent questions relevant to transporters.

References

Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
May 24, 1990·Biochimica Et Biophysica Acta·I Ahmed, G Krishnamoorthy
May 1, 1990·The Journal of Membrane Biology·S H White, R E Jacobs
Jan 1, 1989·Annual Review of Physiology·S A Baldwin, P J Henderson
May 1, 1973·Analytical Biochemistry·P W Holloway
Sep 18, 1980·Biochimica Et Biophysica Acta·T M AllenJ P Segrest
Jan 1, 1995·Journal of Biomolecular NMR·K M LeeJ D Baleja
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·P J SpoonerP J Henderson
Mar 1, 1996·Molecular Microbiology·B PoolmanI Mus-Veteau
Apr 1, 1997·Journal of Structural Biology·J L RigaudJ L Ranck
Aug 4, 1998·European Journal of Biochemistry·M SchwaigerH Kessler
Nov 25, 1998·Biophysical Journal·P J SpoonerA Watts
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·K MadinY Endo
Jun 9, 2000·Molecular Microbiology·M PutmanW N Konings
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chad M RienstraRobert G Griffin
Sep 11, 2002·Angewandte Chemie·David D LawsAlexej Jerschow
Sep 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Misha SoskineShimon Schuldiner
Oct 3, 2002·Protein Expression and Purification·Tara L WinstoneRaymond J Turner

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Citations

Mar 11, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Ernst PetzingerRobert Tampé
May 21, 2009·Biological Chemistry·Ute A Hellmich, Clemens Glaubitz
Apr 7, 2007·The Journal of Antimicrobial Chemotherapy·Gerda SzakonyiPeter J F Henderson
Apr 11, 2008·Acta Biochimica Et Biophysica Sinica·Hao Xie
Nov 1, 2008·Coordination Chemistry Reviews·Luca Ronconi, Peter J Sadler

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