Investigation of 2,6-diisopropylphenol (propofol)-evoked Ca2+ movement and cell death in human glioblastoma cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Wei-Zhe LiangCheng Hsien Lu

Abstract

This study examined whether propofol altered [Ca(2+)](i) and caused cell death in DBTRG-05MG cells. Propofol at 400-1000μM increased [Ca(2+)](i) in a concentration-dependent manner. The signal was decreased partially by removal of extracellular Ca(2+). Propofol-induced Ca(2+) influx was not altered by nifedipine, econazole, SK&F96365, and protein kinase C (PKC) activators; but was inhibited by PKC inhibitor. In Ca(2+)-free medium, pretreatment with the endoplasmic reticulum Ca(2+) pump inhibitors thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) nearly abolished propofol-induced [Ca(2+)](i) rise. Incubation with propofol inhibited thapsigargin or BHQ-induced [Ca(2+)](i) rise. Inhibition of phospholipase C (PLC) with U73122 abolished propofol-induced [Ca(2+)](i) rise. At 300-700μM, propofol killed cells in a concentration-dependent manner. The cytotoxic effect of propofol was partly reversed by prechelating cytosolic Ca(2+) with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). Annexin V/PI staining further showed that 300-500μM propofol evoked apoptosis. Propofol also increased reactive oxygen species (ROS) production. Overall, propofol induced a [Ca(2+)](i) rise by inducing PLC- and PKC-dependent Ca(2+) relea...Continue Reading

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Citations

Jan 22, 2014·European Journal of Pharmacology·Ai NakajimaKatsuji Oguchi
Apr 30, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ayumi KonnoTakehiko Iijima
May 31, 2019·Current Opinion in Anaesthesiology·Junichi SaitoDaqing Ma
May 4, 2021·Current Opinion in Anaesthesiology·Julia DubowitzBernhard Riedel

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