Abstract
The objectives were to study a possible outbreak of carbapenem-resistant Acinetobacter baumannii by comparing three different typing methods (PFGE, MLST and whole-genome SNPs) and to compare the resistance gene profiles of the isolates. From December 2012 to October 2013, eight carbapenem-resistant A. baumannii were detected at Odense University Hospital, Odense, Denmark. These isolates were typed by PFGE, with ApaI and SmaI, respectively, and subjected to WGS. The WGS data were used for in silico extraction of MLST types using two different schemes, resistance genes and SNPs, to which 31 publicly available A. baumannii genomes were added. Using ApaI, the eight isolates had four different PFGE profiles, which were further differentiated using SmaI, separating one of the profiles into two distinct PFGE types. Five ST2 (Pasteur MLST) OXA-23-producing isolates, two ST1 OXA-72-producing isolates and one ST158 OXA-23-producing isolate were detected. The five ST2 isolates were subdivided into ST195, ST208 and ST218 using the Oxford MLST scheme. The phylogenetic analysis based on the core genome showed that six of the eight Danish A. baumannii isolates were located in three distinct clusters. The two remaining isolates did not cluster...Continue Reading
References
Sep 1, 1995·Journal of Clinical Microbiology·F C TenoverB Swaminathan
Mar 28, 2006·International Journal of Antimicrobial Agents·Neil WoodfordDavid M Livermore
Aug 3, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L Poirel, P Nordmann
Mar 30, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A TsakrisK Bethimouti
Sep 2, 2009·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Sep 23, 2009·Antimicrobial Agents and Chemotherapy·Paul G HigginsHarald Seifert
Dec 22, 2009·International Journal of Antimicrobial Agents·Paul G HigginsHarald Seifert
Dec 18, 2010·Journal of Medical Microbiology·Nabil KarahØrjan Samuelsen
Jan 13, 2012·Journal of Clinical Microbiology·Mette V LarsenOle Lund
Mar 27, 2012·The Journal of Antimicrobial Chemotherapy·Shiro EndoMitsuo Kaku
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Nov 7, 2012·International Journal of Antimicrobial Agents·Raffaele ZarrilliAthanassios Tsakris
Feb 23, 2013·The Lancet Infectious Diseases·Johan van GriensvenAsrat Hailu
Feb 27, 2013·Antimicrobial Agents and Chemotherapy·Paul G HigginsHarald Seifert
Jun 1, 2013·BMC Infectious Diseases·Tatsuya TadaTeruo Kirikae
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Citations
Nov 1, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sherief El-ShazlyAshraf S Ibrahim
Aug 24, 2016·Frontiers in Microbiology·Somdatta ChatterjeeSulagna Basu
Nov 5, 2016·Diagnostic Microbiology and Infectious Disease·J Kristie JohnsonKerri A Thom
Sep 12, 2016·Burns : Journal of the International Society for Burn Injuries·Guangtao HuangYizhi Peng
Feb 22, 2018·Antimicrobial Agents and Chemotherapy·Daniel WibbergAndreas Schlüter
Jun 24, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F Tagini, G Greub
Sep 1, 2017·Clinical Microbiology Reviews·Scott QuainooHeiman F L Wertheim
Jul 22, 2015·Genome Biology·Agnes P ChanDerrick E Fouts
Nov 25, 2016·Frontiers in Microbiology·Guangtao HuangYizhi Peng
Aug 24, 2019·Frontiers in Cellular and Infection Microbiology·Chen ChenWen-Hong Zhang
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Xiufeng ZhangWeibiao Lv
May 1, 2021·Microorganisms·Blenda Gonçalves CabralKarla Valéria Batista Lima