Investigation of a possible outbreak of carbapenem-resistant Acinetobacter baumannii in Odense, Denmark using PFGE, MLST and whole-genome-based SNPs

The Journal of Antimicrobial Chemotherapy
Anette M HammerumUlrik S Justesen

Abstract

The objectives were to study a possible outbreak of carbapenem-resistant Acinetobacter baumannii by comparing three different typing methods (PFGE, MLST and whole-genome SNPs) and to compare the resistance gene profiles of the isolates. From December 2012 to October 2013, eight carbapenem-resistant A. baumannii were detected at Odense University Hospital, Odense, Denmark. These isolates were typed by PFGE, with ApaI and SmaI, respectively, and subjected to WGS. The WGS data were used for in silico extraction of MLST types using two different schemes, resistance genes and SNPs, to which 31 publicly available A. baumannii genomes were added. Using ApaI, the eight isolates had four different PFGE profiles, which were further differentiated using SmaI, separating one of the profiles into two distinct PFGE types. Five ST2 (Pasteur MLST) OXA-23-producing isolates, two ST1 OXA-72-producing isolates and one ST158 OXA-23-producing isolate were detected. The five ST2 isolates were subdivided into ST195, ST208 and ST218 using the Oxford MLST scheme. The phylogenetic analysis based on the core genome showed that six of the eight Danish A. baumannii isolates were located in three distinct clusters. The two remaining isolates did not cluster...Continue Reading

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Citations

Nov 1, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sherief El-ShazlyAshraf S Ibrahim
Nov 5, 2016·Diagnostic Microbiology and Infectious Disease·J Kristie JohnsonKerri A Thom
Sep 12, 2016·Burns : Journal of the International Society for Burn Injuries·Guangtao HuangYizhi Peng
Jun 24, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F Tagini, G Greub
Sep 1, 2017·Clinical Microbiology Reviews·Scott QuainooHeiman F L Wertheim
Aug 24, 2019·Frontiers in Cellular and Infection Microbiology·Chen ChenWen-Hong Zhang
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Xiufeng ZhangWeibiao Lv

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